Microcarrier culture: Applications in biological production and cell biology
Microcarrier culture: Applications in biological production and cell biology
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微载体培养：在生物生产和细胞生物学中的应用
DOI：
10.1002/bit.260231204
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发表时间：
1981
影响因子：
3.8
通讯作者：
D. Giard
中科院分区：
文献类型：
--
作者：
C. Crespi;T. Imamura;P. Leong;R. Fleischaker;H. Brunengraber;W. Thilly;D. Giard
本文综述了马萨诸塞州理工学院微载体技术的最新进展。这一进展包括对高细胞密度微载体培养物的营养需求的新理解，在补充激素的无血清培养基中微载体上细胞生长的证明，以及通过允许有效珠到珠转移的培养基修饰在微载体培养物中上皮细胞类型的连续细胞繁殖。此外，报道了通过从微载体上选择性分离获得大量有丝分裂细胞的技术。最后，干扰素生产的最新进展，从人包皮成纤维细胞生长在微载体上概述：我们的干扰素“超级诱导”过程中的改进，每个细胞的干扰素产量增加了5倍至10倍。
This report reviews recent progress in micro carrier technology at the Massachusetts Institute of Technology. This progress includes new understanding of the nutritional needs of high‐cell density micro carrier cultures, the demonstration of cell growth on microcarriers in a hormone‐supplemented, serum‐free medium, and the continuous cell propagation of epithelial cell types in microcarrier culture by medium modification that permits efficient bead‐to bead transfer. Also, a technique for obtaining large quantities of mitotic cells by selective detachment from microcarriers is reported. Finally, recent progress in interferon production from human foreskin fibroblasts grown on microcarriers is outlined: Our improvements in the interferon “super induction” process have increased the interferon yield per cell fivefold to tenfold.