Comprehensive Analysis of the CDPK-SnRK Superfamily Genes in Chinese Cabbage and Its Evolutionary Implications in Plants.
Comprehensive Analysis of the CDPK-SnRK Superfamily Genes in Chinese Cabbage and Its Evolutionary Implications in Plants.
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大白菜CDPK-SnRK超家族基因综合分析及其植物进化意义
DOI：
10.3389/fpls.2017.00162
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发表时间：
2017
影响因子：
5.6
通讯作者：
Hou X
中科院分区：
文献类型：
--
作者：
Wu P;Wang W;Duan W;Li Y;Hou X
越来越多的证据表明，CDPK-SnRK(钙依赖蛋白激酶/Snf1相关蛋白激酶)基因超家族在抗病和各种应激反应的信号通路中发挥着重要作用。本研究对白菜CDPK-SnRK基因的结构、保留、扩增、全基因组复制(WGD)和表达模式及其在植物中的进化进行了比较分析。白菜中共鉴定出49个BrCPK、14个BrCRK、3个BrPPCK、5个BrPEPRK和56个BrSnRK。所有BrCDPK-SnRK蛋白都具有高度保守的激酶域。通过对每个物种中CDPK-SnRK基因数量的统计分析，我们发现CDPK-SnRK基因家族的扩展始于被子植物。CDPK-SnRK基因扩增以节段性复制为主。分析表明，PEPRK比其他亚家族更优先保留，CPK的保留与SnRK相似。在CPK和SnRK中，CPKIII和SnRK1基因比其他组更优先保留。CRK与CPK最接近，可能具有共同的进化起源。此外，我们还在6个物种中鉴定了196个CPK基因和252个SnRK基因，并发现了它们不同的扩展和进化类型。此外，BrCDPK-SnRK基因在不同组织中的表达以及对非生物胁迫的响应是动态的，显示了它们在白菜发育中的重要作用。综上所述，本研究为油菜全基因组三倍体后CDPK-SnRK基因的进化历史和机制提供了全基因组的洞察力。
The CDPK-SnRK (calcium-dependent protein kinase/Snf1-related protein kinase) gene superfamily plays important roles in signaling pathways for disease resistance and various stress responses, as indicated by emerging evidence. In this study, we constructed comparative analyses of gene structure, retention, expansion, whole-genome duplication (WGD) and expression patterns of CDPK-SnRK genes in Brassica rapa and their evolution in plants. A total of 49 BrCPKs, 14 BrCRKs, 3 BrPPCKs, 5 BrPEPRKs, and 56 BrSnRKs were identified in B. rapa. All BrCDPK-SnRK proteins had highly conserved kinase domains. By statistical analysis of the number of CDPK-SnRK genes in each species, we found that the expansion of the CDPK-SnRK gene family started from angiosperms. Segmental duplication played a predominant role in CDPK-SnRK gene expansion. The analysis showed that PEPRK was more preferentially retained than other subfamilies and that CPK was retained similarly to SnRK. Among the CPKs and SnRKs, CPKIII and SnRK1 genes were more preferentially retained than other groups. CRK was closest to CPK, which may share a common evolutionary origin. In addition, we identified 196 CPK genes and 252 SnRK genes in 6 species, and their different expansion and evolution types were discovered. Furthermore, the expression of BrCDPK-SnRK genes is dynamic in different tissues as well as in response to abiotic stresses, demonstrating their important roles in development in B. rapa. In summary, this study provides genome-wide insight into the evolutionary history and mechanisms of CDPK-SnRK genes following whole-genome triplication in B. rapa.