Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116
Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116
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Aurantiochytrium 物种的分离和表征：新分离的微藻 Aurantiochytrium sp SD116 产生高二十二碳六烯酸 (DHA)
DOI：
10.5650/jos.62.143
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发表时间：
2013-03-01
影响因子：
1.5
通讯作者：
Cui, Qiu
中科院分区：
文献类型：
--
作者：
Gao, Mang;Song, Xiaojin;Cui, Qiu
从广东省水东湾海域分离到一株高产二十二碳六烯酸（DHA，C22：6 n-3）的异养微藻，命名为SD 116。对SD 116菌株的18 S rDNA序列分析表明，该菌株与金壶菌属（Aurantiochytrium）的亲缘关系较近。SD 116在6.0%葡萄糖、2.0%酵母提取物和50%人工海水（ASW）中获得最高的生长和DHA积累速率，pH为7，28 ℃。总脂含量最高可达细胞干重的56.3%，DHA含量最高可达总脂肪酸的50.9%。进一步发现，尿素由于其便宜的价格和诱导相对高的生物量和脂质生产能力的能力，可能是用于工业发酵的潜在氮源。使用5 L发酵罐，发现DCW、总脂质含量和DHA产量分别为70.43 g L-1、DCW的71.09%和17.42 g L-1（TFA的34.79%）。研究结果表明，橙色壶菌SD 116是一株有希望的DHA商业化生产菌株，可用于生物质相关产品的合成。
A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis of the 18S rDNA of SD116 showed that the strain has a close phylogenetic relationship to Aurantiochytrium species. The highest rates for growth and DHA accumulation for SD116 were obtained in 6.0% glucose, 2.0% yeast extract, and 50% artificial seawater (ASW) at a pH of 7 at 28 degrees C. The maximum total lipid content reached 56.3% of the dry cell weight (DCW), and the maximum DHA content accounted for 50.9% of the total fatty acid (TFA) content. It was further found that urea may be a potential nitrogen source for industrial fermentation because of its cheap price and ability to induce a relatively high biomass and lipid production capacity. Using 5 L fermenters, the DCW, total lipid content, and DHA yield were found to be 70.43 g L-1, 71.09% of the DCW, and 17.42 g L-1 (34.79% of the TFA), respectively. The results show that Aurantiochytrium sp. SD116 is a promising candidate for commercial DHA production and could be useful for the synthesis of biomass-related products.