Type I and III collagen protein precursors and mRNA in the developing human lung
Type I and III collagen protein precursors and mRNA in the developing human lung
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DOI：
10.1002/path.1547
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发表时间：
2004-05-01
影响因子：
7.3
通讯作者：
Soini, Y
中科院分区：
文献类型：
--
作者：
Kaarteenaho-Wiik, R;Pääkkö, P;Soini, Y
细胞外基质蛋白在人肺的个体发生和纤维化中具有突出的作用。本研究的目的是分析新形成的前体蛋白和mRNA的胶原蛋白I型和III型在发育中的人肺组织从妊娠12至40周，也在新生儿疾病，如呼吸窘迫综合征（RDS）和支气管肺发育不良（BPD）的表达。尸检时从60例非畸形病例中获得肺组织。所有组织进行了分析，免疫组化和24也进行了研究，mRNA原位杂交。两种胶原的前体蛋白和mRNA在肺动脉和肺静脉的各个发育时期都有大量表达。在RDS和BPD中，肺泡壁内两种胶原类型的前体蛋白和mRNA增加。这些位置的细胞显示α-平滑肌肌动蛋白、波形蛋白和可变结蛋白免疫反应性。胶原蛋白I和III前体蛋白和mRNA也观察到胸膜，支气管，细支气管，和周围的软骨细胞在所有的发展时期，并在患病的肺。总之，胶原蛋白I和III在发育中的肺中的各种细胞类型中和周围以相似的方式表达，并且它们在RDS和BPD的肺泡壁内的表达增加。肌纤维母细胞型细胞似乎产生mRNA的两种类型的胶原蛋白在肺泡。版权所有（C）2004大不列颠和爱尔兰病理学会。出版社：John Wiley Sons，Ltd
Extracellular matrix proteins have a prominent role in both ontogenesis and fibrogenesis in the human lung. The aim of this study was to analyse the expression of newly formed precursor proteins and mRNA of collagen types I and III in developing human lung tissues from 12 to 40 weeks of gestation, and also in neonatal disorders such as respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD). Lung tissues were obtained at autopsy from 60 non-malformed cases. All tissues were analysed by immunohistochemistry and 24 were also investigated by mRNA in situ hybridization. The precursor proteins and mRNA of both collagens were expressed in abundance in pulmonary arteries and veins during all developmental periods. In RDS and BPD, precursor proteins and mRNAs of both collagen types were increased within alveolar walls. The cells in these locations showed a-smooth muscle actin, vimentin, and variable desmin immunoreactivity. Collagen I and III precursor proteins and mRNA were also observed in pleura, bronchi, bronchioles, and around chondrocytes during all developmental periods and in diseased lung. In conclusion, collagens I and III were expressed in a similar way in and around various cell types in the developing lung and their expression was increased within alveolar walls in RDS and BPD. Myofibroblast-type cells appeared to produce mRNA for both types of collagen in alveoli. Copyright (C) 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley Sons, Ltd.