A functional quantitative polymerase chain reaction assay for ricin, Shiga toxin, and related ribosome-inactivating proteins
A functional quantitative polymerase chain reaction assay for ricin, Shiga toxin, and related ribosome-inactivating proteins
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DOI：
10.1016/j.ab.2009.09.024
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发表时间：
2010-01-15
影响因子：
2.9
通讯作者：
Tolleson, William H.
中科院分区：
文献类型：
--
作者：
Melchior, William B., Jr.;Tolleson, William H.
蓖麻毒素、蓖麻毒素和其他使核糖体失活的蛋白质使28S核糖体RNA中的一个特定碱基死基化，从而破坏核糖体并导致细胞死亡。我们利用逆转录酶优先在RNA中插入与基本位点相反的腺嘌呤这一事实，创建了定量聚合酶链反应（PCR）试验来检测损伤。这种检测方法只检测到30 pg的蓖麻毒素。我们用该方法研究了蓖麻毒素的酶学性质，如pH和温度的最佳值（pH 4.5-5.0和60℃）。Elsevier Inc.出版。
The potent toxins ricin, abrin, and other ribosome-inactivating proteins deadenylate a specific base in 28S ribosomal RNA that destroys ribosomes and leads to cell death. We have taken advantage of the fact that reverse transcriptase preferentially inserts an adenine opposite to an abasic site in RNA to create a quantitative polymerase chain reaction (PCR) assay to detect the damage. This assay detects as little as 30 pg of ricin. We used the assay to study enzymatic properties of ricin such as pH and temperature optima (pH 4.5-5.0 and 60 degrees C). Published by Elsevier Inc.