Effects of exogenous nitric oxide on photosynthesis, antioxidative ability, and mineral element contents of perennial ryegrass under copper stress
Effects of exogenous nitric oxide on photosynthesis, antioxidative ability, and mineral element contents of perennial ryegrass under copper stress
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DOI：
10.1080/17429145.2013.845917
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发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
Bai, Xiaoying
中科院分区：
文献类型：
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作者：
Dong, Yuanjie;Xu, Linlin;Bai, Xiaoying
采用水培试验研究了不同浓度硝普钠（SNP）对铜（Cu）毒害作用的影响。过量的铜（200亩M）降低叶绿素含量，导致光合作用下降。Cu胁迫诱导过氧化氢（H2 O2）和超氧阴离子（O2-）的产生，导致丙二醛（MDA）的积累。此外，铜处理的幼苗的抗氧化酶，如超氧化物歧化酶（SOD），过氧化物酶（POD），过氧化氢酶（CAT）的活性下降。此外，Cu胁迫抑制了根中K、Mg、Fe和Zn的吸收，增加了根中Ca的含量。Cu胁迫下，叶片中K、Fe、Zn含量降低，Ca、Mg含量无明显变化。在Cu处理的幼苗中，根中Cu浓度高于叶。在铜介导的溶液中添加50、100、200 μ M的SNP可提高叶绿素含量和光合作用，提高抗氧化酶活性，减轻铜诱导的氧化损伤，维持铜胁迫下的细胞内离子平衡，提高根中铜浓度，抑制叶片中铜的积累。特别是在Cu胁迫下，添加100 μ M SNP对促进黑麦草幼苗生长的效果最好。但400亩SNP对铜毒害的缓解效果不明显。
A hydroponics experiment was conducted to test the effects of sodium nitroprusside (SNP, a donor of NO) supplied with different concentrations on copper (Cu) toxicity in ryegrass seedlings (Lolium perenne L.). Excess Cu (200 mu M) reduced chlorophyll content, resulting a decrease in photosynthesis. Cu stress induced the production of hydrogen peroxide (H2O2) and superoxide anion (O-2(center dot-)), leading to malondialdehyde (MDA) accumulation. Furthermore, activities of antioxidant enzymes in Cu-treated seedlings such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were decreased. In addition, Cu stress inhibited the uptake of K, Mg, Fe, and Zn and increased Ca content in roots. Moreover, in leaves of Cu-stressed seedlings, K, Fe, and Zn contents were decreased and the contents of Ca and Mg were not affected significantly. In Cu-treated seedlings, Cu concentration in roots was higher than in leaves. Addition of 50, 100, 200 mu M SNP in Cu-mediated solutions increased chlorophyll content and photosynthesis, improved antioxidant enzyme activities, reduced Cu-induced oxidative damages, kept intracellular ion equilibrium under Cu stress, increased Cu concentration in roots and inhibited Cu accumulation in leaves. In particular, addition of 100 mu M SNP had the best effect on promoting growth of ryegrass seedlings under Cu stress. However, the application of 400 mu M SNP had no obvious alleviating effect on Cu toxicity in ryegrass seedlings.