Evaluation of AMCase and CHIT-1 expression in monocyte macrophages lineage
Evaluation of AMCase and CHIT-1 expression in monocyte macrophages lineage
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DOI：
10.1007/s11010-012-1506-5
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发表时间：
2013-02-01
影响因子：
4.3
通讯作者：
Malaguarnera, Lucia
中科院分区：
文献类型：
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作者：
Di Rosa, Michelino;De Gregorio, Corinne;Malaguarnera, Lucia
酸性哺乳动物几丁质酶(AMCase)和几丁糖三糖苷酶(Chit-1)是人类表达的两种具有活性的几丁质酶。AMCase的几丁质酶活性被发现与变态反应性炎症有关，其表达被白介素13诱导。CHIT1-1由吞噬细胞表达，在溶酶体储存性疾病中水平极高。尽管AMCase在炎症中的表达仍在研究中，但对其在巨噬细胞充分成熟和极化过程中的调控知之甚少。在这项研究中，我们比较了AMCase和Chit-1在单核细胞向巨噬细胞转变和极化过程中的调节。采用实时荧光定量聚合酶链式反应(Real-time PCR)技术，对健康志愿者外周血中单核细胞、干扰素(IFN)-γ和脂多糖(LPS)处理后极化激活的巨噬细胞(M1)和白介素4(IL-4)刺激的交替激活的巨噬细胞(M2)的mRNA进行了基因表达分析。结果表明，AMCase和Chit-1在不同成熟阶段HMM中的表达受到不同程度的调控。这两种活性几丁质酶的行为表明，在免疫反应中，它们的作用是互补的。
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. Despite that AMCase expression in the inflammation is under investigation, little is known regarding its regulation during macrophages' full maturation and polarization. In this study, we compared AMCase and CHIT-1 modulation during monocyte to macrophage transition and polarization. Gene expression analysis was investigated by real-time PCR from mRNA of human monocytes obtained from buffy coat of healthy volunteers, from mRNA of polarized to classically activated macrophages (or M1), obtained by interferon (IFN)-gamma and lipopolysaccharide (LPS) treatment, and from mRNA of alternatively activated macrophages (or M2) obtained by interleukin (IL)-4 exposure. Our results showed that the expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation. The behavior of these two active chitinase suggests that in the immune response their role is complementary.