PREGNANCY DIAGNOSIS IN SOWS BASED ON SERUM ESTRONE SULFATE CONCENTRATION
PREGNANCY DIAGNOSIS IN SOWS BASED ON SERUM ESTRONE SULFATE CONCENTRATION
复制标题
DOI：
10.1136/vr.113.11.229
复制
发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
WRATHALL, AE
中科院分区：
文献类型：
--
作者：
CUNNINGHAM, NF;HATTERSLEY, JJP;WRATHALL, AE
对4个品种(大白猪、长白猪、大白猪、长白猪和帝王杂交猪)1275头母猪交配后25~30天的血清硫酸雌酮浓度进行了测定。用一种简单、直接的20微升血清放射免疫法测定硫酸雌酮，根据血清中硫酸雌酮含量超过0.5 ng/ml表示怀孕母猪，小于0.5 ng/ml表示未怀孕进行妊娠诊断。根据硫酸雌酮诊断怀孕的总体准确率为98%；这不受品种或采样日期的影响(在测试范围内)。对于妊娠母猪，血清雌酮硫酸盐水平与产仔数呈正相关，但个体值不能用来预测特定母猪的产仔数。在妊娠的最后1/3阶段，从怀孕的帝王母猪身上采集的样品中也检测到了雌酮硫酸盐的浓度，在第77天或以后采集的样品中，99%的样品中的雌酮含量超过0.5 ng/ml。因此，测定交配后76天以上样本的血清硫酸雌酮水平可作为妊娠的确证试验。
Serum oestrone sulphate concentration was measured in samples taken from 1275 sows of four breeds or crossbreeds (large white, landrace, large white cross landrace and monarch hybrid) during the period 25 to 30 days after mating. A simple, direct radioimmunoassay using 20 microliter serum was employed for the estimation of oestrone sulphate and pregnancy diagnoses were made on the basis that more than 0.5 ng oestrone sulphate/ml serum indicated a pregnant sow and less than 0.5 ng/ml indicated non-pregnancy. Overall accuracy of pregnancy diagnosis based on oestrone sulphate was 98 per cent; this was not influenced by breed or day sampled (within the range tested). For pregnant sows, there was a positive correlation between serum oestrone sulphate level and litter size, although individual values could not be used to predict litter size for particular sows. Oestrone sulphate concentration was also measured in samples taken from pregnant monarch sows during the last third of the gestation period and the level was more than 0.5 ng/ml in 99 per cent of samples taken on day 77 or later. Thus, measurement of serum oestrone sulphate level in samples taken more than 76 days after mating could be used as a confirmatory test of pregnancy.