Layer-by-Layer Assembly of Metal-Organic Frameworks in Macroporous Polymer Monolith and Their Use for Enzyme Immobilization
Layer-by-Layer Assembly of Metal-Organic Frameworks in Macroporous Polymer Monolith and Their Use for Enzyme Immobilization
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大孔聚合物整体中金属有机框架的层层组装及其在酶固定化中的应用
DOI：
10.1002/marc.201500705
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发表时间：
2016
影响因子：
4.6
通讯作者：
Lv Yongqin
中科院分区：
文献类型：
--
作者：
Wen Liyin;Gao Aicong;Cao Yao;Svec Frantisek;Tan Tianwei;Lv Yongqin
通过层层自组装策略制备了包含沸石咪唑酯骨架（ZIF-8）的新型整体材料，该材料位于先前用N-（3-氨基丙基）-咪唑功能化的聚（甲基丙烯酸缩水甘油酯-共聚-二甲基丙烯酸乙二醇酯）整体材料的孔表面上。这些新的 ZIF-8@monolith 杂化物用作酶固定化的固相载体。它们的性能通过模型蛋白水解酶胰蛋白酶的固定得到证明。最好的缀合物能够非常有效地消化蛋白质，只需 43 秒即可实现。
New monolithic materials comprising zeolitic imidazolate framework (ZIF‐8) located on the pore surface of poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monolith previously functionalized withN‐(3‐aminopropyl)‐imidazole have been prepared via a layer‐by‐layer self‐assembly strategy. These new ZIF‐8@monolith hybrids are used as solid‐phase carriers for enzyme immobilization. Their performance is demonstrated with immobilization of a model proteolytic enzyme trypsin. The best of the conjugates enable very efficient digestion of proteins that can be achieved in mere 43 s.