Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium.
Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium.
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DOI：
10.1210/endo-111-3-919
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发表时间：
1982-09
期刊：
影响因子：
4.8
通讯作者：
M. H. Simonian;M. White;G. Gill
中科院分区：
文献类型：
--
作者：
M. H. Simonian;M. White;G. Gill
一种无血清确定的培养基，支持细胞增殖和分化功能的类固醇生成已被开发为单层培养的正常牛肾上腺皮质细胞。细胞培养物在纤连蛋白包被的基质上从低细胞密度（1-2 × 103个细胞/cm 2）增殖，培养基由Ham F-12和Dulbecco改良Eagle培养基的1：1混合物组成，补充有4 nM成纤维细胞生长因子、2 nM胰岛素、100 mU/ml凝血酶、10μ g/ml低密度脂蛋白、100 μ g/ml转铁蛋白、500 μg/ml无脂肪酸BSA、100 μM抗坏血酸、1 μM α-生育酚和50 nM硒。成分确定培养基中的细胞培养物以与补充有血清和成纤维细胞生长因子的培养基中的细胞培养物相同的生长速率增殖。在完全不存在血清的情况下，肾上腺皮质细胞在限定培养基中连续传代三次，直至达到相同的最终细胞密度。细胞增殖在确定的培养基中被完全抑制ACTH，这是以前的。
A serum-free defined medium that supports cellular proliferation and the differentiated function of steroidogenesis has been developed for monolayer cultures of normal bovine adrenocortical cells. Cell cultures proliferate from a low cell density (1–2 × 103 cells/cm2) on a fibronectin-coated substratum in a medium composed of a 1:1 mixture of Ham's F-12 and Dulbecco's Modified Eagle's Medium supplemented with 4 nM fibroblast growth factor, 2 nM insulin, 100 mU/ml thrombin, 10μg/ml low density lipoprotein, 100 μg/ml transferrin, 500 μg/ml fatty acid-free BSA, 100 μM ascorbic acid, 1 μM α-tocopherol, and 50 nM selenium. The cell cultures in the defined medium proliferate at the same growth rate as those in medium supplemented with serum and fibroblast growth factor. In the total absence of serum, the adrenocortical cells have been grown in the defined medium for three consecutive passages to the same final cell density. Cell proliferation in the defined medium was completely inhibited by ACTH, which previou...