In Vitro S. epidermidis and S. aureus Adherence to Composite and Lightweight Polypropylene Grafts
In Vitro S. epidermidis and S. aureus Adherence to Composite and Lightweight Polypropylene Grafts
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DOI：
10.1016/j.jss.2009.04.008
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发表时间：
2009-11-01
影响因子：
2.2
通讯作者：
Vural, Tayfun
中科院分区：
文献类型：
--
作者：
Aydinuraz, Kuzey;Agalar, Canan;Vural, Tayfun
背景尽管存在显著的感染风险，但假体移植材料已成为切口疝修补术不可或缺的材料。复合材料和轻质移植物已被开发出来，以克服粘连和刚性。本研究的目的是评价体外S。epidermidis和S.金黄色葡萄球菌粘附到这些新一代移植物上，这些新一代移植物具有改性的组成和纺织品性质。在本实验研究中，将多丝聚丙烯、多丝轻质复合材料、单丝复合材料和单丝聚丙烯移植物的无菌样品与粘液阳性S.表皮葡萄球菌和表皮葡萄球菌。金黄色葡萄球菌菌株24 h。将移植物在盐水中洗涤并涡旋。将盐水传代至血琼脂并孵育24 h。对菌落进行计数。通过扫描电子显微镜检查幼稚和孵育的移植材料，以揭示纺织品性质和生物膜形成。评价了理化性质。采用Wilcoxon符号秩和检验和Friedman检验进行统计学分析。S.表皮对单丝聚丙烯移植物的粘附性明显降低，而对多丝聚丙烯移植物的粘附性明显增加（P
Background. Despite the significant risk of infection, prosthetic graft materials have become indispensable for incisional hernia repair. Composite and lightweight grafts have been developed to overcome adhesion and rigidity. The aim of this study was to evaluate in vitro S. epidermidis and S. aureus adherence to these new generation grafts, which have modified composition and textile properties.Materials and Methods. In this experimental study, sterile samples of multifilament polypropylene, multifilament lightweight composite, monofilament composite, and monofilament polypropylene grafts were incubated with slime positive S. epidermidis and S. aureus strains for 24 h. The grafts were washed and vortexed in saline. The saline was passaged to blood agar and incubated for 24 h. The colonies were counted. Naive and incubated graft materials were examined by scanning electron microscopy to reveal both textile properties and biofilm formation. Physicochemical properties were evaluated. Wilcoxon signed ranks test and Friedman test were used for statistical analysis.Results. S. epidermidis showed a significantly decreased adherence to monofilament polypropylene graft, and increased adherence to multifilament polypropylene graft (P