Impact of chicory inclusion in a cereal-based diet on digestibility, organ size and faecal microbiota in growing pigs
Impact of chicory inclusion in a cereal-based diet on digestibility, organ size and faecal microbiota in growing pigs
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DOI：
10.1017/s1751731111002709
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发表时间：
2012-07-01
期刊：
影响因子：
3.6
通讯作者：
Lindberg, J. E.
中科院分区：
文献类型：
--
作者：
Ivarsson, E.;Liu, H. Y.;Lindberg, J. E.
选用30头7周龄猪，研究菊苣包埋对消化率、消化器官大小和粪便微生物群的影响。试验配制5种饲粮：以谷物为基础的对照饲粮和添加80和160 g/kg菊苣饲料（CF80和CF160）、80 g/kg菊苣根（CR80）以及80 g/kg饲料和80 g/kg菊苣根（CFR）的混合饲粮。总体而言，猪的生长速度和采食量较高，不同日粮之间没有差异。能量、有机物和粗蛋白质的全肠道表观消化率（CTTAD）系数在对照组和CF80之间无显著差异，而在饲粮CF160中有所降低。菊苣包合组非淀粉多糖的CTTAD显著高于对照组（P < 0.05），其中以CF160组最高（P < 0.05）。饲粮CFR组大肠菌群计数低于对照组（P < 0.05），乳酸菌与大肠菌群比值高于对照组（P < 0.05）。采用末端限制性片段长度多态性、克隆和测序相结合的方法研究全球微生物组成。肠道菌群模式分析显示，CF160日粮与对照组和CR80日粮存在两大差异。菊苣饲料与一种普氏菌相关物种的相对丰度增加相关，与另外两种普氏菌相关物种的相对丰度降低相关。饲料CFR中约氏乳杆菌的相对丰度高于其他饲料。本研究表明，菊苣草料和菊苣根均可作为猪营养中的纤维来源，并且它们对肠道菌群组成的调节不同。
A total of 30 7-week-old pigs were used to evaluate the effects of chicory inclusion on digestibility, digestive organ size and faecal microbiota. Five diets were formulated: a cereal-based control diet and four diets with inclusion of 80 and 160 g/kg chicory forage (CF80 and CF160), 80 g/kg chicory root (CR80) and a mix of 80 g/kg forage and 80 g/kg chicory root (CFR). Generally, the pigs showed a high growth rate and feed intake, and no differences between the different diets were observed. The coefficients of total tract apparent digestibility (CTTAD) of energy, organic matter and CP did not differ between the control and CF80, whereas they were impaired in diet CF160. The CTTAD of non-starch polysaccharides and especially the uronic acids were higher (P < 0.05) with chicory inclusion, with highest (P < 0.05) values for diet CF160. Coliform counts were lower and lactobacilli: coliform ratio was higher (P < 0.05) in diet CFR than in the control. Global microbial composition was investigated by terminal restriction fragment length polymorphism combined with cloning and sequencing. Analysis of gut microbiota pattern revealed two major clusters where diet CF160 differed from the control and CR80 diet. Chicory forage diets were correlated with an increased relative abundance of one species related to Prevotella and decreased abundance of two other species related to Prevotella. For diet CFR, the relative abundance of Lactobacillus johnsonii was higher than in the other diets. This study shows that both chicory forage and root can be used as fibre sources in pig nutrition and that they modulate the composition of the gut microbiota differently.