Rat lung glucose metabolism after 24 h of exposure to 100% oxygen.
Rat lung glucose metabolism after 24 h of exposure to 100% oxygen.
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大鼠%20肺%20葡萄糖%20代谢%20after%2024%20h%20of%20暴露%20to%20100%%20氧气。
DOI：
10.1152/jappl.1989.66.2.989
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Reichenbaugh,SS
中科院分区：
文献类型：
--
作者：
Bassett,DJ;Bowen-Kelly,E;Reichenbaugh,SS
先前对肺匀浆和分离细胞的研究表明，氧细胞损伤是由于参与细胞质和线粒体能量生成的关键酶受到抑制所致。在本研究中，我们检测了大鼠离体灌注肺在体内暴露于100% O2 24 h前后D-[U-14C， 5-3H]葡萄糖代谢的程度和模式。葡萄糖利用率从未暴露的对照值18.0 +/- 3.2增加到27.5 +/- 3.0 μ mol 3H2O.h-1，增加了53%，维持了氧暴露后肺ATP水平。G干wt-1，乳酸和丙酮酸产率从15.7 +/- 2.0提高到32.7 +/- 4.1 mmol .h-1。G干wt-1，乳酸与丙酮酸比值无变化。CO2产量在控制速率为27.5 +/- 4.0 14CO2 mol / h-1的基础上保持不变。G干wt-1。通过灌注0.8 mM二硝基苯酚测定最大糖代谢率，暴露于空气中的肺的糖代谢率为53.5 +/- 5.0 μ mol 3H2O.h-1。使乳酸+丙酮酸和14CO2的产率分别提高了46.5 +/- 6.5和128.3 +/- 19.6 mmol .h-1。分别是G干wt-1。尽管氧暴露肺的最大葡萄糖利用率没有改变，乳酸和丙酮酸的产量进一步增加到80.0 +/- 9.1 mmol .h-1。同时，二硝基酚诱导的14CO2产率降至81.5 +/- 9.2 mmol .h-1。G干wt-1。（摘要删节250字）
Previous studies with lung homogenates and isolated cells have suggested oxygen cell injury results from the inhibition of key enzymes involved in both cytosolic and mitochondrial energy generation. In this study, the extent and pattern of metabolism of D-[U-14C, 5–3H]glucose was examined in perfused lungs isolated from rats before and after 24 h of in vivo exposure to 100% O2. Lung ATP levels after O2 exposure were maintained by a 53% increase in glucose utilization from an unexposed control value of 18.0 +/- 3.2 to 27.5 +/- 3.0 mumol 3H2O.h-1.g dry wt-1, accounted for by an enhanced rate of lactate plus pyruvate production from 15.7 +/- 2.0 to 32.7 +/- 4.1 mumol.h-1.g dry wt-1 with no alteration in lactate-to-pyruvate ratio. CO2 production was unaltered from a control rate of 27.5 +/- 4.0 14CO2 mumol.h-1.g dry wt-1. Maximal rates of glucose metabolism were determined by perfusion with 0.8 mM dinitrophenol, giving for air-exposed lungs a rate of 53.5 +/- 5.0 mumol 3H2O.h-1.g dry wt-1 and increased lactate plus pyruvate and 14CO2 production rates of 46.5 +/- 6.5 and 128.3 +/- 19.6 mumol.h-1.g dry wt-1, respectively. Although this maximal rate of glucose utilization was unaltered in oxygen-exposed lungs, lactate plus pyruvate production was further increased to 80.0 +/- 9.1 mumol.h-1.g dry wt-1 with a concomitant decrease in the dinitrophenol-induced rate of 14CO2 production to 81.5 +/- 9.2 mumol.h-1.g dry wt-1.(ABSTRACT TRUNCATED AT 250 WORDS)