Variations in Endosymbiont Infection Between Buprofezin-Resistant and Susceptible Strains of Laodelphax striatellus (Fall,n)
Variations in Endosymbiont Infection Between Buprofezin-Resistant and Susceptible Strains of Laodelphax striatellus (Fall,n)
复制标题
灰飞虱耐药和敏感菌株之间内共生体感染的变化（Fall,n）
DOI：
10.1007/s00284-018-1436-x
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发表时间：
2018-06-01
影响因子：
2.6
通讯作者：
Guo, Huifang
中科院分区：
文献类型：
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作者：
Li, Yongteng;Liu, Xiangdong;Guo, Huifang
内共生菌沃尔巴克氏体和立克次体已被证明与蚊子和粉虱的杀虫剂抗性有关。小褐飞虱（ladelphax striatellus）是一种内共生植物，对丁丙霉嗪具有较高的抗性。本研究检测了丁丙非津耐药（BR）菌株、丁丙非津敏感（BS）菌株和丁丙非津暴露后的BR菌株内共生菌的种类和感染发生率，并研究了从BR菌株中去除沃尔巴克氏体后丁丙非津敏感性的变化。结果表明，BR和BS菌株的沃尔巴克氏体感染率均为100%，但BR菌株的沃尔巴克氏体密度显著高于BS菌株。两菌株的毒Arsenophonus感染发生率无显著差异。然而，BR菌株的沙雷氏菌感染发生率和沙雷氏菌+沃尔巴克氏菌双重感染发生率显著高于BS菌株。1200mg /L buprofezin处理后，BR菌株存活个体中Arsenophonus的感染率升高，而Serratia的感染率无差异，但Serratia + Wolbachia的双重感染发生率降低。当来自BR菌株的沃尔巴克氏体感染系清除沃尔巴克氏体后，其对丁丙非津的敏感性增加。结果提示，沙雷氏菌和沃尔巴克氏菌感染可能会改善SBPH对丁丙非津的耐药性。
The endosymbionts Wolbachia and Rickettsia have been shown to be correlated with the insecticide resistance of mosquito and whitefly. The small brown planthopper (SBPH), Laodelphax striatellus, harbours many species of endosymbionts, and has developed a high resistance to buprofezin in China. In this study, we examined the species and the infection incidences of endosymbionts in a buprofezin-resistant (BR) strain, a buprofezin-susceptible (BS) strain, and the BR strain after exposure to buprofezin, and we also investigated the change in buprofezin susceptibility after removal of Wolbachia from the BR strain. The results showed that Wolbachia infection incidences were 100% in both the BR and BS strains, but the Wolbachia density in the BR strain was significantly higher than that in the BS strain. There were no significant differences in Arsenophonus infection incidence between the two strains. However, the infection incidence of Serratia and double infection incidence of Serratia + Wolbachia in the BR strain were significantly higher than that in the BS strain. After the BR strain was exposed to 1200 mg/L buprofezin, the infection incidence of Arsenophonus in the surviving individuals increased, and the infection rate of Serratia did not differ, but the double infection incidence of Serratia + Wolbachia decreased. And when a Wolbachia-infected line originating from the BR strain was cleared of Wolbachia, its susceptibility to buprofezin increased. The results suggest that Serratia and Wolbachia infection might improve the buprofezin resistance of SBPH.