Hierarchical structure assembly model of rice dwarf virus particle formation.
Hierarchical structure assembly model of rice dwarf virus particle formation.
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DOI：
10.1007/s12551-017-0375-2
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发表时间：
2018-04-01
影响因子：
--
通讯作者：
Higashiura, Akifumi
中科院分区：
文献类型：
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作者：
Nakagawa, Atsushi;Miyazaki, Naoyuki;Higashiura, Akifumi
水稻矮缩病毒（Rice dwarf virus，RDV）是呼肠孤病毒科（Reoviridae）和植物呼肠孤病毒属（Phytoreovirus）的成员，是引起水稻矮缩病的原因，水稻矮缩病是包括日本在内的整个东亚地区水稻生产的主要负效应物。RDV具有直径约70nm的二十面体双层壳。构成衣壳的结构蛋白可以在体外自组装成正确的颗粒，而不需要任何外部因素的帮助。总共有900多个组件组装成完整的粒子。一系列的结构和功能的RDV的研究，包括X射线晶体学和冷冻电子显微镜，提出了一个层次的自组装机制，涉及同源和异源相互作用。因此，用于识别每个组分的系统对于颗粒形成是必不可少的。
Rice dwarf virus (RDV) of the family Reoviridae and genus Phytoreovirus, is the cause of rice dwarf disease, a major negative effector of rice production throughout East Asia, including Japan. RDV has an icosahedral double-layered shell of approximately 70nm diameter. The structural proteins constituting the capsid can self-assemble into a correct particle without requiring the help of any external factors in vitro. A total of more than 900 components assemble to make the full particle. A series of structural and functional studies of RDV, including X-ray crystallography and cryo-electron microscopy, suggest a hierarchical self-assembly mechanism involving both homologous and heterologous interactions. As such, systems for the recognition of each component should be essential for particle formation.