Differential expression of LeY and fucosyltransferase IV correlates with the receptivity of RL95-2 and HEC-1A human uterine epithelial cells
Differential expression of LeY and fucosyltransferase IV correlates with the receptivity of RL95-2 and HEC-1A human uterine epithelial cells
复制标题
LeY和岩藻糖基转移酶IV的差异表达与RL95-2和HEC-1A人子宫上皮细胞的接受性相关
DOI：
10.1042/cbi20100644
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发表时间：
2012-05-01
影响因子：
3.9
通讯作者：
Yan, Qiu
中科院分区：
文献类型：
--
作者：
Liu, Shuai;Yang, Xuesong;Yan, Qiu
子宫内膜上表达的粘附分子是胚胎着床的潜在受体标记。RL 95 -2和HEC-1A细胞系分别代表高和低接受性子宫内膜上皮。LeY（刘易斯Y）是一种双岩藻糖基化低聚糖，在某些物种的着床期子宫内膜中高度表达。LeY的α 1，3岩藻糖基化由FUT 4（岩藻糖基转移酶IV）催化，FUT 4是LeY的关键合成酶。我们研究了两种细胞系之间的感受性差异是否与LeY和FUT 4的不同表达有关。免疫荧光染色、RT-PCR（reverse transcription-PCR）和Western blotting显示，RL 95 -2细胞表达LeY和FUT 4的水平高于HEC-1A细胞。FUT 4-siRNA转染可下调RL 95 -2细胞中FUT 4和LeY的表达，并抑制胚胎细胞与单层细胞的粘附。FUT 4-cDNA可增加HEC-1A细胞中FUT 4和LeY的表达，并增加胚胎细胞与HEC-1A细胞单层的粘附。通过上调或下调FUT 4引起的LeY水平的改变也介导了EGFR（表皮生长因子受体）/MAPK（促分裂原活化蛋白激酶）信号通路。结论：LeY和FUT 4的表达与子宫内膜容受性相关，可作为评价子宫内膜容受性的新指标。
Adhesion molecules expressed on the uterine endometrium are potential receptive markers in embryo implantation. RL95-2 and HEC-1A cell lines represent the high- and low-receptive endometrial epithelium respectively. LeY (Lewis Y) is a difucosylated oligosaccharide highly expressed in the endometrium of some species during implantation. alpha 1, 3 fucosylation of LeY is catalysed by FUT4 (fucosyltransferase IV), the key synthesis enzyme for LeY. We investigated whether the difference in receptivity between the 2 cell lines was related to different expressions of LeY and FUT4. RL95-2 cells expressed a higher level of LeY and FUT4 than HEC-1A cells, as shown by immunofluorescent staining, RT-PCR (reverse transcription-PCR) or Western blotting. FUT4-siRNA (small interfering RNA) transfection down-regulated FUT4 and LeY in RL95-2 cells, and inhibited the adhesion of the embryonic cells (JAR) to RL95-2 cell monolayer. FUT4-cDNA, however, increased the expression of FUT4 and LeY in HEC-1A cells, and increased the adhesion of embryonic cells to HEC-1A cell monolayer. Alterations of LeY level by up- or down-regulation of FUT4 also mediated EGFR (epidermal growth factor receptor)/MAPK (mitogen-activated protein kinase) signalling pathway. To conclude, the expression of LeY and FUT4 correlates with endometrial receptivity, making them potential new markers for the evaluation of endometrial receptivity.