Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomyces coelicolor A3(2)
Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomyces coelicolor A3(2)
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DOI：
10.1016/j.febslet.2014.09.037
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发表时间：
2014-11
期刊：
影响因子：
3.5
通讯作者：
Takeshi Hayashi;Yoshikazu Tanaka;N. Sakai;U. Okada;M. Yao;N. Watanabe;T. Tamura;I. Tanaka
中科院分区：
文献类型：
--
作者：
Takeshi Hayashi;Yoshikazu Tanaka;N. Sakai;U. Okada;M. Yao;N. Watanabe;T. Tamura;I. Tanaka
SCO7518是一种来自colelicolorstreptomyces coelicola3(2)的功能未知的蛋白，已被归类为TetR转录调控家族。在本研究中，以2.29 Å分辨率测定了SCO7518的晶体结构。该结构是原聚体的同二聚体，包括n端dna结合域和c端二聚化和调节域，并具有假定的配体结合腔。配体的基因组系统进化通过指数富集和电泳迁移转移分析显示，SCO7518特异性结合位于co7519基因上游的一个操作序列，该序列编码麦芽糖o -乙酰转移酶。这些结果表明SCO7518是sco7519表达的转录抑制因子。
SCO7518 is a protein of unknown function fromStreptomyces coelicolorA3(2) that has been classified into the TetR transcriptional regulator family. In this study, a crystal structure of SCO7518 was determined at 2.29 Å resolution. The structure is a homodimer of protomers that comprise an N-terminal DNA-binding domain and a C-terminal dimerization and regulatory domain, and possess a putative ligand-binding cavity. Genomic systematic evolution of ligands by exponential enrichment and electrophoretic mobility shift assays revealed that SCO7518 specifically binds to an operator sequence located upstream of thesco7519gene, which encodes a maltose O-acetyltransferase. These results suggest that SCO7518 is a transcriptional repressor ofsco7519expression.