Zinc Finger-Homeodomain Genes: Evolution, Functional Differentiation, and Expression Profiling Under Flowering-Related Treatments and Abiotic Stresses in Plants
Zinc Finger-Homeodomain Genes: Evolution, Functional Differentiation, and Expression Profiling Under Flowering-Related Treatments and Abiotic Stresses in Plants
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DOI：
10.1177/1176934319867930
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发表时间：
2019-09-05
影响因子：
2.6
通讯作者：
An, Na
中科院分区：
文献类型：
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作者：
Shalmani, Abdullah;Muhammad, Izhar;An, Na
锌指同源结构域(ZHD)蛋白是一个植物特有的转录因子家族，在植物的生长、发育和逆境反应中发挥重要作用。在本研究中，我们分别研究了桃、拟南芥和苹果基因组中的10个、17个和31个ZHD基因成员。系统发育树根据ZHD基因的结构域组织、基因结构和基序分布将其划分为4个亚家族，变异较小。ZHD基因家族成员在苹果、桃和拟南芥基因组中分布不均。在苹果中观察到14对基因的节段性重复。转录分析发现，ZHD基因主要在各种组织中表达，特别是在叶片和花中。此外，大多数ZHD基因的转录在不同的时间点都受到与开花相关的外源激素(糖、赤霉素[GA]和6-苄氨基嘌呤[6-BA])的显著影响，这表明它们可能在苹果开花诱导中发挥作用。此外，CaZHD6、CaZHD7、CaZHD3和CaZHD8的转录本已经诱导了对热、干旱、盐和冷等非生物胁迫的响应，这表明它们可能参与了对非生物胁迫的响应。我们的研究工作系统地展示了ZHD基因的不同作用。我们相信，本研究将为ZHD基因未来的功能鉴定提供平台，并深入揭示其在植物开花诱导中的调控作用。
Zinc finger-homeodomain (ZHD) proteins constitute a plant-specific transcription factor family that play important roles in plant growth, development, and stress responses. In this study, we investigated a total of 10, 17, and 31 ZHD gene members in the peach, Arabidopsis, and apple genome, respectively. The phylogenetic tree divided the identified ZHD genes into 4 subfamilies based on their domain organization, gene structure, and motif distribution with minor variations. The ZHD gene family members were unevenly distributed throughout in apple, peach, and Arabidopsis genomes. Segmental duplication was observed for 14 pairs of genes in apple. Transcript analysis found that ZHD genes mostly expressed in various tissues, particularly in leaves and flowers. Moreover, the transcript of most ZHD genes was significantly affected at different time points in response to various flowering-related exogenous hormones (sugar, gibberellin [GA], and 6-benzylaminopurine [6-BA]), signifying their possible role in the flowering induction in apple. Furthermore, the transcripts of CaZHD6, CaZHD7, CaZHD3, and CaZHD8 have induced in response to abiotic stresses including heat, drought, salt, and cold, indicating their possible involvement in response to abiotic stresses. Our research work systemically presents the different roles of ZHD genes. We believe that this study will provide a platform for future functional characterization of ZHD genes and to deeply unfold their roles in the regulation of flowering induction in plants.