Characterization of integrin expression in the mouse ovary
Characterization of integrin expression in the mouse ovary
复制标题
DOI：
10.1095/biolreprod.101.000729
复制
发表时间：
2002-09-01
影响因子：
3.6
通讯作者：
Matzuk, MM
中科院分区：
文献类型：
--
作者：
Burns, KH;Owens, GE;Matzuk, MM
整合素帽子异源二聚体介导细胞与细胞外基质的接触，并启动细胞内信号级联反应，以响应各种因素。整合素与细胞表型的许多决定因素相互作用，并在控制每种类型组织的发育、结构完整性和功能方面发挥作用。尽管它们很重要，但对哺乳动物卵巢中整合素亚单位的调节以及它们在卵泡发生中的作用知之甚少。为了确定它们与卵巢生理的相关性，我们用Northern印迹分析和原位杂交的方法研究了整合素亚单位mRNAs在野生型、生长分化因子9(GDF9)基因敲除、FSHB(FSHB)基因敲除和抑制素α(InHA)基因敲除小鼠卵巢中的表达。整合素ET、mRNA表达于单层卵泡的卵母细胞和颗粒细胞，表达于多层卵泡的卵母细胞和卵泡膜细胞。整合素α(6)在GDF9基因敲除的卵巢中高度表达，由于在卵泡发育的这个阶段受阻，这些卵巢富含在卵母细胞和初级(单层)卵泡中。整合素α(V)在多层生长卵泡的颗粒细胞中表达最高，因此只有在GDF9基因敲除的卵巢中才能检测到低水平的表达。整合素β(1)在卵巢中广泛表达，包括卵母细胞、颗粒细胞、卵泡膜细胞和黄体。整合素β(3)在卵泡膜细胞和间质细胞中表达，在黄体中表达上调。在FSHB基因敲除小鼠的卵巢中几乎检测不到它，这些小鼠有腔前卵泡，但没有黄体细胞。整合素β(5)，mRNA主要在多层卵泡的颗粒细胞中表达。它在FSHB基因敲除小鼠中高水平表达，在InhA基因敲除小鼠发展中的颗粒细胞/支持细胞肿瘤中以区隔方式表达。在小鼠中，特定的整合素与卵巢细胞表型相关，这增加了关于整合素在卵母细胞能力、卵泡发育、黄体化和颗粒细胞增殖中的作用的有趣的可能性。
Integrin cap heterodimers mediate cell contacts to the extracellular matrix and initiate intracellular signaling cascades in response to a variety of factors. Integrins interact with many determinants of cellular phenotypes and play roles in controlling the development, structural integrity, and function of every type of tissue. Despite their importance, little is known about the regulation of integrin subunits in the mammalian ovary and how they function in folliculogenesis. To determine their relevance to ovarian physiology, we have studied the expression of integrin subunit mRNAs by Northern blot analysis and in situ hybridization in ovaries of wild-type, growth differentiation factor 9 (Gdf9) knockout, FSHbeta (Fshb) knockout, and inhibin alpha (Inha) knockout mice. Integrin et, mRNA is expressed in oocytes and granulosa cells of single-layer follicles and in oocytes and theca cells of multilayer follicles. Integrin alpha(6) is highly expressed in Gdf9 knockout ovaries, which are enriched in oocytes and primary (single layer) follicles because of a block at this stage of follicular development. Integrin alpha(v), mRNA is most highly expressed in the granulosa cells of multilayer growing follicles, and therefore only low levels of expression are detectable in the Gdf9 knockout ovaries. Integrin beta(1) mRNA exhibits a broad expression pattern in ovaries, including oocytes, granulosa cells, theca cells, and corpora lutea. Integrin beta(3) mRNA is expressed in theca and interstitial cells and is upregulated in corpora lutea. It is nearly undetectable in ovaries of Fshb knockout mice, which develop preantral follicles but have no luteal cells. Integrin beta(5), mRNA is predominantly expressed in granulosa cells of multilayer follicles. It is expressed at high levels in the Fshb knockout mice and in a compartmentalized manner in the granulosa cell/Sertoli cell tumors that develop in the Inha knockout mice. Specific integrins are associated with ovarian cellular phenotypes in mice, which raises intriguing possibilities as to integrin functions in oocyte competence, follicular development, luteinization, and granulosa cell proliferation.