MISTRANSLATION IN IGF-1 DURING OVER-EXPRESSION OF THE PROTEIN IN ESCHERICHIA-COLI USING A SYNTHETIC GENE CONTAINING LOW-FREQUENCY CODONS
MISTRANSLATION IN IGF-1 DURING OVER-EXPRESSION OF THE PROTEIN IN ESCHERICHIA-COLI USING A SYNTHETIC GENE CONTAINING LOW-FREQUENCY CODONS
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DOI：
10.1016/s0006-291x(88)81117-3
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发表时间：
1988-08-30
影响因子：
3.1
通讯作者：
SIEGEL, NR
中科院分区：
文献类型：
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作者：
SEETHARAM, R;HEEREN, RA;SIEGEL, NR
当使用合成基因在大肠杆菌中表达IGF-1时，观察到IGF-1的Arg残基的部分Lys错误掺入Arg，所述合成基因具有编码所有六个Arg残基的低频阿加密码子和编码剩余残基的酵母优选密码子。当含有E.大肠杆菌优选的密码子，密码子CGT编码所有的Arg残基，用于蛋白质的表达。类似地，当使用在所有位置都含有大肠杆菌偏好密码子的基因时，除了在Arg（36）处的阿加密码子，不能检测到Arg的Lys错误掺入。
Partial misincorporation of Lys for Arg has been observed for the Arg residues of IGF-1 when the molecule is expressed in Escherichia coli using a synthetic gene with the low frequency AGA codon encoding all six Arg residues and yeast preferred codons encoding the remaining residues. The Lys for Arg substitution at these residues could not be detected when a gene containing E. coli preferred codons, with the codon CGT coding for all Arg residues, was used for the expression of the protein. Similarly, no misincorporation of Lys for Arg could be detected when a gene containing Escherichia coli preferred codons at all positions, except for an AGA codon at Arg (36), was utilized.