Plasma membrane H+ and K+ transporters are involved in the weak-acid preservative response of disparate food spoilage yeasts
Plasma membrane H+ and K+ transporters are involved in the weak-acid preservative response of disparate food spoilage yeasts
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DOI：
10.1099/mic.0.27502-0
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发表时间：
2005-06-01
期刊：
影响因子：
2.8
通讯作者：
Davies, JM
中科院分区：
文献类型：
--
作者：
Macpherson, N;Shabala, L;Davies, JM
食品腐败酵母菌Zygosaccharomyces bailii和Saccharomyces cerevisiae通过不同的方式抵抗弱酸性防腐剂胁迫，Zygosaccharomyces bailii通过限制防腐剂的流入结合其催化剂，S.通过对防腐剂弱酸性阴离子和H+的活性挤出，指数相Z bailii细胞H+挤出的测量表明，与S。在酿酒酵母中，当受到弱酸防腐剂（苯甲酸）的挑战时，这种酵母使用质膜H+-ATP酶来排出H+。同时测量百利藻的H+和K+净通量表明，在急性苯甲酸胁迫期间，K+净流入伴随着H+净流出。这种离子耦合对于S是已知的。酿酒酵母在短期防腐应力。这两种酵母显着积累K+长期暴露于苯甲酸。分析S.酿酒酵母K+转运蛋白突变体的研究表明，高亲和力K+摄取系统Trk 1的丧失赋予了对防腐剂中生长的敏感性。结果表明，阳离子积累是腐败酵母适应弱酸性防腐剂的一个重要因素;酿酒酵母在质膜离子转运水平上共享迄今未被怀疑的适应性反应。
The food spoilage yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae have been proposed to resist weak-acid preservative stress by different means; Z bailii by limiting influx of preservative combined with its catabolism, S. cerevisiae by active extrusion of the preservative weak-acid anion and H+. Measurement of H+ extrusion by exponential-phase Z bailii cells suggest that, in common with S. cerevisiae, this yeast uses a plasma membrane H+-ATPase to expel H+ when challenged by weak-acid preservative (benzoic acid). Simultaneous measurement of Z bailii net H+ and K+ fluxes showed that net K+ influx accompanies net H+ efflux during acute benzoic acid stress. Such ionic coupling is known for S. cerevisiae in short-term preservative stress. Both yeasts significantly accumulated K+ on long-term exposure to benzoic acid. Analysis of S. cerevisiae K+ transporter mutants revealed that loss of the high affinity K+ uptake system Trk1 confers sensitivity to growth in preservative. The results suggest that cation accumulation is an important factor in adaptation to weak-acid preservatives by spoilage yeasts and that Z bailii and S. cerevisiae share hitherto unsuspected adaptive responses at the level of plasma membrane ion transport.