Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses
Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses
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DOI：
10.1099/0022-1317-78-9-2293
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发表时间：
1997-09-01
影响因子：
3.8
通讯作者：
Deubel, V
中科院分区：
文献类型：
--
作者：
Berthet, FX;Zeller, HG;Deubel, V
我们比较了从9个非洲国家和法国分离的21个时间上不同的西尼罗河（WN）病毒株的包膜蛋白基因片段的序列。核苷酸序列的比对定义了两组病毒，它们的差异高达29%。第一组亚型由来自法国和非洲的9种WN菌株组成。在第一组中，澳大利亚-亚洲昆津病毒被归类为WN亚型。第二组包括来自非洲和马达加斯加的12个WN菌株。有4个菌株携带12个核苷酸的框内缺失。相应的四个氨基酸的损失导致在几个WN菌株中存在的潜在糖基化位点的损失。病毒亚型分为两个谱系的分布与宿主偏好或地理来源无关。在遥远的国家分离到密切相关的亚型与迁徙鸟类传播的WN病毒一致。
We compared the sequence of an envelope protein gene fragment from 21 temporally distinct West Nile (WN) virus strains, isolated in nine African countries and in France. Alignment of nucleotide sequences defined two groups of viruses which diverged by up to 29%. The first group of subtypes is composed of nine WN strains from France and Africa. The Austral-Asian Kunjin virus was classified as a WN subtype in this first group. The second group includes 12 WN strains from Africa and Madagascar. Four strains harboured a 12 nucleotide in-frame deletion. The loss of the corresponding four amino acids resulted in the loss of the potential glycosylation site present in several WN strains. The distribution of virus subtypes into two lineages did not correlate with host preference or geographical origin. The isolation of closely related subtypes in distant countries is consistent with WN viruses being disseminated by migrating birds.