Study on the DNA Barcoding of Genus Ligularia Cass. (Asteraceae)
Study on the DNA Barcoding of Genus Ligularia Cass. (Asteraceae)
复制标题
DOI：
10.7677/ynzwyj201515082
复制
发表时间：
2015-11-25
期刊：
影响因子：
--
通讯作者：
Pan Yue-zhi
中科院分区：
文献类型：
--
作者：
He Wei-ying;Pan Yue-zhi
DNA条形码技术是一种基于短而标准化的DNA序列快速鉴定物种的新技术。橐吾属菊科的一个属，约有140种。具有高度的形态和生态多样性。这使得分类和物种界定变得困难。特别是在密切相关的分类群的情况下。本研究我们测试了四个DNA核心条形码区（ITS. matK。psbA-trnH和rbcL）。结果表明，叶绿体区（matK. psbA-trnH和rbcL）种间变异小，种间鉴定率极低。相反。ITS序列具有较高的种间鉴别率（60%），并能区分出难以鉴别的种。这四个基因片段的组合并没有提高物种辨别能力。
DNA barcoding is a new technology which can identify species rapidly based on short and standardized DNA sequences. Ligularia. a genus of Asteraceae with about 140 species. exhibits high morphological and ecological diversity. which makes the classification and species delimitation difficult. especially in the cases of closely related taxa. In this study. we tested four DNA core barcoding regions (ITS. matK. psbA-trnH and rbcL) in 144 samples representing 35 species of Ligularia. The results revealed that the chloroplast regions (matK. psbA-trnH and rbcL) have extremely low species identification rate due to low interspecific variation. Conversely. ITS sequence showed higher species identification rate (60%) and could discriminate the species which are difficult to identify. The combination of these four gene fragments did not improve the ability of species discrimination.