Mutations in rpoB gene of rifampicin resistant clinical isolates of Mycobacterium tuberculosis in Japan.
Mutations in rpoB gene of rifampicin resistant clinical isolates of Mycobacterium tuberculosis in Japan.
复制标题
日本耐利福平结核分枝杆菌临床分离株 rpoB 基因突变
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
M. Makino
中科院分区：
文献类型：
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作者：
Yasuhiko Suzuki;C. Katsukawa;Kiyoshi Inoue;Yue Ping Yin;Hironobu Tasaka;Noboru Ueba;M. Makino
在结核分枝杆菌中，Telenti等人描述了RNA聚合酶β亚单位的改变与利福平耐药性的关系。结核分枝杆菌利福平耐药表型在日本，47株M。结核病的利福平耐药表型，17个利福平敏感表型和4个模式菌株进行了检查。通过聚合酶链反应扩增411个碱基对（bp）的rpoB片段，并进行固相直接测序。通过核苷酸比较，47株利福平耐药株中有44株（93.6%）发生了8个保守氨基酸的突变，而17株敏感株和4株典型株均未发生突变。发现的所有突变都聚集在23个氨基酸的区域内。因此，与Telenti等人报告的结果相似，由rpoB基因编码的有限数量的高度保守氨基酸的取代似乎是引起日本M临床分离株对利福平耐药的分子机制。结核我们的研究结果表明，rpoB基因的直接DNA测序可能是一种可靠的方法来确定利福平耐药的M。日本临床分离株中的结核菌株。
In Mycobacterium tuberculosis, involvement of alterations of the RNA polymerase beta subunit in resistance to rifampicin has been described by Telenti et al. To determine if the same correlation could be observed between the mutation of the rpoB gene and clinically isolated M. tuberculosis of the rifampicin-resistant phenotype in Japan, 47 strains of M. tuberculosis of the rifampicin-resistant phenotype, 17 of the rifampicin-susceptible phenotype, and 4 type strains were examined. A 411-base pair (bp) rpoB fragment was amplified by the polymerase chain reaction and subjected to solid phase direct sequencing. By comparing the nucleotides, mutation involving 8 conserved amino acids were identified in 44 of the 47 (93.6%) rifampicin-resistant isolates, but in none of the 17 sensitive isolates and 4 type strains. All mutations found were clustered within a region of 23 amino acids. Thus, similar to the results reported by Telenti et al., substitution of a limited number of highly conserved amino acids encoded by the rpoB gene appears to be the molecular mechanism responsible for resistance to rifampicin in Japanese clinical isolates of M. tuberculosis. Our results suggest that direct DNA sequencing of the rpoB gene may be a reliable method for identifying rifampicin-resistant M. tuberculosis strains among Japanese clinical isolates.
影响因子：
56.9
作者：
BANERJEE, A;DUBNAU, E;JACOBS, WR
通讯作者：
JACOBS, WR