DEOXYRIBONUCLEIC ACID-DEPENDENT RIBONUCLEIC ACID POLYMERASE ACTIVITY IN CELLS INFECTED WITH INFLUENZA VIRUS
DEOXYRIBONUCLEIC ACID-DEPENDENT RIBONUCLEIC ACID POLYMERASE ACTIVITY IN CELLS INFECTED WITH INFLUENZA VIRUS
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DOI：
10.1128/jvi.2.1.33-39.1968
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发表时间：
1968-01-01
影响因子：
5.4
通讯作者：
MAHY, BWJ
中科院分区：
文献类型：
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作者：
BORLAND, R;MAHY, BWJ
用甲型流感病毒(鸡瘟病毒)感染鸡胚成纤维细胞，在不同时间检测细胞核DNA依赖的核糖核酸(RNA)聚合酶活性，并与未感染细胞的活性进行比较。聚合酶活性在2小时内增加了约60%。在感染后，这种增加与受感染细胞的RNA合成增加相一致，这是通过尿苷脉冲标记确定的。感染细胞和未感染细胞的聚合酶对DNA引物、二价阳离子和核侧三磷酸盐的需求没有差异，而且它们对反应混合物中添加放线菌素D同样敏感。宿主细胞DNA依赖的RNA聚合酶可能参与流感病毒RNA的复制。
Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase activity was assayed on nuclear preparations of chick embryo fibroblast cells at various times after infection with an influenza A virus (fowl plague virus) and was compared with the activity of uninfected cells. Polymerase activity was increased by about 60% by 2 hr. after infection, and this increase coincided with an increase in RNA synthesis in infected cells, as determined by pulse-labeling with uridine. No differences could be detected between the polymerases of infected and uninfected cells as to their requirements for DNA primer, divalent cations, and nucleo-side triphosphates, and they were equally sensitive to addition of actinomycin D to the reaction mixture. It is possible that host cell DNA-dependent RNA polymerase is involved in the replication of-influenza virus RNA,.