ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane
ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane
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DOI：
10.1038/nsmb998
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发表时间：
2005-11-01
影响因子：
16.8
通讯作者：
Beckmann, R
中科院分区：
文献类型：
--
作者：
Blau, M;Mullapudi, S;Beckmann, R
翻译分泌和膜蛋白的核糖体靶向内质网膜，并附着于蛋白传导通道和核糖体相关膜蛋白（RAMP）。最近，一个新的RAMP，ERj 1 p，已被确定，招募BiP核糖体和调节翻译活性。在这里，我们提出的cryo-EM结构的核糖体ERj 1 p复合物，揭示ERj 1 p如何协调核糖体在膜上，以及如何变构效应可能介导ERj 1 p的调节活性。
Ribosomes translating secretory and membrane proteins are targeted to the endoplasmic reticulum membrane and attach to the protein-conducting channel and ribosome-associated membrane proteins (RAMPs). Recently, a new RAMP, ERj1p, has been identified that recruits BiP to ribosomes and regulates translational activity. Here we present the cryo-EM structure of a ribosome-ERj1p complex, revealing how ERj1p coordinates the ribosome at the membrane and how allosteric effects may mediate ERj1p's regulatory activity.