Effect of spirulina on the secretion of cytokines from peripheral blood mononuclear cells.
Effect of spirulina on the secretion of cytokines from peripheral blood mononuclear cells.
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DOI：
10.1089/jmf.2000.3.135
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发表时间：
2000-01-01
影响因子：
2.4
通讯作者：
Gershwin, M E
中科院分区：
文献类型：
--
作者：
Mao, T K;VAN DE Water, J;Gershwin, M E
摘要本研究的目的是评估螺旋藻，一种蓝绿色植物作为食品补充剂的免疫调节活性。研究了螺旋藻对人外周血单个核细胞（PBMC）分泌三种细胞因子的影响。在静止的PBMC中，螺旋藻刺激白细胞介素（IL）-1 β，IL-4和干扰素（IFN）-γ的分泌，分别接近基础水平的2.0，3.3和13.6倍。螺旋藻诱导的IFN-γ水平（229 +/- 104 pg/ml）与植物血凝素（PHA）刺激后观察到的水平（476 +/- 121 pg/ml）相当。然而，就诱导IL-4分泌（0.34 +/- 0.1 pg/ml）而言，其促有丝分裂作用比PHA（13.1 +/- 6.9 pg/ml）低得多。在PHA刺激的细胞中，螺旋藻使IL-1 β、IL-4和IFN-β的分泌分别增加2.9、4.0.和1.6倍。虽然螺旋藻刺激几种细胞因子，但它显然在产生Th 1型应答方面更有效。这项体外研究为考虑螺旋藻的潜在治疗益处提供了额外的数据。
ABSTRACT The purpose of this study was to evaluate the immunomodulatory activity of Spirulina, a bluegreen alga used as a food supplement. The effects of Spirulina on the secretion of three cytokines from unstimulated and stimulated human peripheral blood mononuclear cells (PBMC) were examined. In resting PBMC, Spirulina stimulated secretion of interleukin (IL)-1beta, IL-4, and interferon (IFN)-gamma to nearly 2.0, 3.3, and 13.6 times basal levels, respectively. Spirulina induced levels of IFN-gamma (229 +/- 104 pg/ml) that were comparable to those seen after phytohemagglutinin (PHA) stimulation (476 +/- 121 pg/ml). However, it was much less mitogenic than PHA (13.1 +/- 6.9 pg/ml) with respect to the induction of IL-4 secretion (0.34 +/- 0.1 pg/ml). In PHA-stimulated cells, Spirulina enhanced secretion of IL-1beta, IL-4, and IFN-beta by 2.9, 4.0., and 1.6 times, respectively. Although Spirulina stimulates several cytokines, it is clearly more effective in the generation of a Thl-type response. This in vitro study offers additional data for consideration of the potential therapeutic benefits of Spirulina.