The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs
The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs
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DOI：
10.1016/s1097-2765(01)00437-3
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发表时间：
2002-01-01
期刊：
影响因子：
16
通讯作者：
Storz, G
中科院分区：
文献类型：
--
作者：
Zhang, AX;Wassarman, KM;Storz, G
大肠杆菌宿主因子 I Hfq 与许多小调节 RNA 结合，并且是 OxyS RNA 抑制 fhIA 和 rpoS mRNA 翻译所必需的。在此，我们报道 Hfq 是真核细胞中 RNA 加工和 mRNA 降解复合物中不可或缺的 Sm 和 Sm 样蛋白的细菌同源物。 Hfq 表现出 Sm 和 Sm 样蛋白的标志性特征：Sm1 序列基序、多亚基环结构（在本例中为同聚六聚体）以及优先与 PolyU 结合。我们还表明，Hfq 增加了 OxyS RNA 与其靶标信息的相互作用，并提出 RNA-RNA 配对的增强可能是 Hfq、Sm 和 Sm 样蛋白的一般功能。
The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhIA and rpoS mRNA translation. Here we report that Hfq is a bacterial homolog of the Sm and Sm-like proteins integral to RNA processing and mRNA degradation complexes in eukaryotic cells. Hfq exhibits the hallmark features of Sm and Sm-like proteins: the Sm1 sequence motif, a multisubunit ring structure (in this case a homomeric hexamer), and preferential binding to polyU. We also show that Hfq increases the OxyS RNA interaction with its target messages and propose that the enhancement of RNA-RNA pairing may be a general function of Hfq, Sm, and Sm-like proteins.