Bladder and cutaneous sensory neurons of the rat express different functional P2X receptors
Bladder and cutaneous sensory neurons of the rat express different functional P2X receptors
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DOI：
10.1016/s0306-4522(03)00243-4
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
McMahon, SB
中科院分区：
文献类型：
--
作者：
Zhong, Y;Banning, AS;McMahon, SB
应用免疫组织化学和膜片钳技术研究了成年大鼠和小鼠膀胱和皮肤感觉神经元P2 X受体的表达和功能反应。将固蓝注射到膀胱肌壁中后，在L 6和S1背根神经节（DRG）中鉴定出膀胱盆腔传入的细胞体。类似地，在将固蓝注射到支配皮肤的隐神经中之后，在L3和L4 DRG中鉴定皮肤感觉神经元。膀胱感觉神经元仅含有弱至中度的P2 X3免疫反应性（IR），而在皮肤传入神经亚群中观察到强的P2 X（3）-IR。全细胞膜片钳记录显示，大约90%的膀胱传入神经元对字母亚甲基ATP（字母表ATP）和ATP（30 μ M）产生持续电流反应，这些电流被2 '，3'-O-三硝基苯基-ATP（TNP-ATP）（0.3 μ M）抑制至对照的6.4 +/- 1.9%和8.0 +/- 2.6%（n=8）。其余的膀胱感觉神经元表现出双相的，短暂的或没有反应的P2 X激动剂。相比之下，只有24%的皮肤传入神经元的持续电流alphabetameATP（30 μ M），66%的细胞给予短暂或双相电流，其余10%无反应。我们的研究结果表明，在一般的DRG神经元，膀胱感觉神经元通过盆神经投射主要表达P2 X（2/3）异聚体受体，这是可能介导的重要作用ATP作为膀胱充盈和伤害性感受的信号分子。(C)2003年IBRO。由Elsevier Science Ltd.出版，版权所有。
The expression and functional responses of P2X receptors in bladder and cutaneous sensory neurons of adult rats and mice have been studied using immunohistochemistry and patch clamp techniques. Cell bodies of bladder pelvic afferents were identified in L6 and S1 dorsal root ganglia (DRG), following Fast Blue injection into the muscle wall of the urinary bladder. Similarly, cutaneous sensory neurons were identified in L3 and L4 DRG, following Fast Blue injection into the saphenous nerve innervating the skin. Bladder sensory neurons contained only weak to moderate P2X3_ immunoreactivity (IR), in contrast to strong P2X(3)-IR observed in a sub-population of cutaneous afferents. Whole-cell patch-clamp recordings revealed that approximately 90% of bladder afferent neurons responded to alphabeta-methylene ATP (alphabetameATP) and ATP (30 muM) with persistent currents, which were inhibited by 2',3'-O-trinitrophenyl-ATP (TNP-ATP) (0.3 muM) to 6.4 +/- 1.9% and 8.0 +/- 2.6% of control, respectively (n=8). The remaining bladder sensory neurons demonstrated biphasic, transient or no response to P2X agonists. In contrast, only 24% of cutaneous afferent neurons gave persistent currents to alphabetameATP (30 muM), with 66% of cells giving transient or biphasic currents and the remaining 10% being nonresponsive. Our results suggest that, in contrast to DRG neurons in general, bladder sensory neurons projecting via pelvic nerves express predominantly P2X(2/3) heteromeric receptors, which are likely to mediate the important roles of ATP as a signaling molecule of urinary bladder filling and nociception. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.