Vertical cross-sectional imaging of colonic dysplasia in vivo with multi-spectral dual axes confocal endomicroscopy.
Vertical cross-sectional imaging of colonic dysplasia in vivo with multi-spectral dual axes confocal endomicroscopy.
复制标题
使用多光谱双轴共焦内窥镜对体内结肠发育不良进行垂直横截面成像。
DOI：
10.1053/j.gastro.2014.01.016
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发表时间：
2014
期刊：
影响因子：
29.4
通讯作者：
Wang,ThomasD
中科院分区：
文献类型：
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作者：
Qiu,Zhen;Khondee,Supang;Duan,Xiyu;Li,Haijun;Mandella,MichaelJ;Joshi,BishnuP;Zhou,Quan;Owens,ScottR;Kurabayashi,Katsuo;Oldham,KennR;Wang,ThomasD
病理学家评估组织学垂直于组织表面的切片，或垂直横截面。这一定位(XZ平面)可以评估基底膜到管腔方向的粘膜分化。目前的内窥镜使用传统的(单轴)光学设计。1成像仅限于水平横截面(XY平面)，其中显微解剖在视场(FOV)中经常是相似的。在双轴结构中，光在轴外传输和收集，图像可以在更大的强度范围内被检测到。2使用荧光收集的分子图像可以提高疾病检测的特异性，并揭示组织的功能属性。3正确解释这些图像需要与显微解剖相关联。我们的目标是演示使用双轴共聚焦内窥镜在体内垂直横截面上同时采集两个荧光图像。来自正常和发育不良小鼠结肠粘膜的分子和解剖图像将被实时显示。
Pathologists evaluate histology sectioned perpendicular to the tissue surface, or vertical cross-section. This orientation (XZ-plane) enables evaluation of mucosal differentiation in the basilar-to-luminal direction. Current endomicroscopes use a conventional (single axis) optical design.1 Imaging is limited to horizontal cross-sections (XY-plane) where the micro-anatomy is frequently similar across the field-of-view (FOV). In the dual axes configuration, light is delivered and collected off-axis, and images can be detected over a much larger range of intensities.2 Molecular images collected using fluorescence can improve specificity for disease detection and reveal functional properties about tissue.3 Proper interpretation of these images requires correlation with the micro-anatomy. We aim to demonstrate the simultaneous collection of two fluorescence images in vivo in vertical cross-sections using a dual axes confocal endomicroscope. An overlay of molecular and anatomical images from normal and dysplastic mouse colonic mucosa will be displayed in real time.