BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm, Bombyx mori
BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm, Bombyx mori
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BmHpo 突变导致家蚕 (Bombyx mori) 体型变小和晚期幼虫致死率
DOI：
10.1111/1744-7917.12607
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发表时间：
2018
期刊：
影响因子：
4
通讯作者：
He Lin
中科院分区：
文献类型：
--
作者：
Xu Xia;Bi Hong Lun;Zhang Zhong Jie;Yang Yang;Li Kai;Huang Yong Ping;Zhang Yong;He Lin
Hpo作为Hippo信号通路的核心成员，在调节生长发育中发挥着关键作用。先前的研究报道，Hpo 功能的丧失会导致果蝇增殖增加、细胞凋亡减少并诱导组织过度生长。在本研究中，我们使用 CRISPR/Cas9（成簇规则间隔短回文重复序列/Cas9）来研究鳞翅目昆虫 Bombyx mori（俗称蚕）的 Hpo 基因 (BmHpo) 功能。 BmHpo 的序列分析揭示了突变体中的一系列缺失。我们发现 BmHpo 敲除会导致体型调节缺陷、发育缺陷、色素积累和早期死亡。我们的数据表明，BmHpo 对于昆虫生长和发育的调控至关重要，CRISPR/Cas9 技术可以作为鳞翅目昆虫靶基因功能分析的基础。
As a core member of the Hippo signaling pathway, Hpo plays a critical role in regulating growth and development. Previous studies reported that loss of function of Hpo results in increased proliferation, reduced apoptosis and induction of tissue overgrowth in Drosophila. In this study, we used CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) to study Hpo gene (BmHpo) function in the lepidopteran insect Bombyx mori, known commonly as the silkworm. Sequence analysis of BmHpo revealed an array of deletions in mutants. We found that BmHpo knockout resulted in defects in body size regulation, in developmental defects and pigment accumulation and early death. Our data show that BmHpo is essential for regulation of insect growth and development and that CRISPR/Cas9 technology can serve as a basis for functional analysis of target genes in lepidopteran insects.