Properties and usefulness of aggregates of synovial mesenchymal stem cells as a source for cartilage regeneration.
Properties and usefulness of aggregates of synovial mesenchymal stem cells as a source for cartilage regeneration.
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DOI：
10.1186/ar3869
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发表时间：
2012-06-07
影响因子：
4.9
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Suzuki S;Muneta T;Tsuji K;Ichinose S;Makino H;Umezawa A;Sekiya I
滑膜间充质干细胞移植是一种很有前途的软骨再生治疗方法。在临床应用中，需要改进操作方法，增强成软骨潜能，提高MSCs的粘附效率，以实现更成功的软骨再生，而不需要支架的MSCs数量有限。使用聚集的MSC可能是解决方案之一。在这里，我们研究了聚集的MSC用于软骨再生的处理、性质和有效性。使用悬滴技术聚集人和兔滑膜MSC。采用基因芯片和真实的实时RT-PCR分析滑膜间充质干细胞聚集后基因表达的变化。在体外和体内的滑膜间充质干细胞聚集体的软骨形成潜力进行了检查。培养三天的MSC聚集体变得可见，直径约为1 mm，并且通过操作是固体和耐用的;大多数细胞是活的。微阵列分析显示，在MSC的聚集体中，软骨形成相关的抗炎和抗凋亡基因上调。体外研究表明，与单层培养的MSC来源的颗粒相比，MSC聚集体来源的颗粒中软骨基质合成量更高。在兔体内实验中，MSCs聚集体可通过表面张力迅速粘附在骨软骨缺损处，且无任何损失。以相对低的密度移植MSC的聚集体实现了成功的软骨再生。与我们的预期相反，以高密度移植MSC聚集体由于细胞死亡和MSC聚集体的营养剥夺而未能再生软骨。滑膜间充质干细胞的聚集是一个有用的来源，考虑到这些因素，易于制备，更高的软骨形成潜力和有效的附着。
Transplantation of mesenchymal stem cells (MSCs) derived from synovium is a promising therapy for cartilage regeneration. For clinical application, improvement of handling operation, enhancement of chondrogenic potential, and increase of MSCs adhesion efficiency are needed to achieve a more successful cartilage regeneration with a limited number of MSCs without scaffold. The use of aggregated MSCs may be one of the solutions. Here, we investigated the handling, properties and effectiveness of aggregated MSCs for cartilage regeneration. Human and rabbit synovial MSCs were aggregated using the hanging drop technique. The gene expression changes after aggregation of synovial MSCs were analyzed by microarray and real time RT-PCR analyses. In vitro and in vivo chondrogenic potential of aggregates of synovial MSCs was examined. Aggregates of MSCs cultured for three days became visible, approximately 1 mm in diameter and solid and durable by manipulation; most of the cells were viable. Microarray analysis revealed up-regulation of chondrogenesis-related, anti-inflammatory and anti-apoptotic genes in aggregates of MSCs. In vitro studies showed higher amounts of cartilage matrix synthesis in pellets derived from aggregates of MSCs compared to pellets derived from MSCs cultured in a monolayer. In in vivo studies in rabbits, aggregates of MSCs could adhere promptly on the osteochondral defects by surface tension, and stay without any loss. Transplantation of aggregates of MSCs at relatively low density achieved successful cartilage regeneration. Contrary to our expectation, transplantation of aggregates of MSCs at high density failed to regenerate cartilage due to cell death and nutrient deprivation of aggregates of MSCs. Aggregated synovial MSCs were a useful source for cartilage regeneration considering such factors as easy preparation, higher chondrogenic potential and efficient attachment.
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影响因子：
2.8
作者：
Niikura, Takahiro;Hak, David J.;Reddi, A. Hari
通讯作者：
Reddi, A. Hari
影响因子：
4
作者：
Shirasawa, S;Sekiya, I;Muneta, T
通讯作者：
Muneta, T
影响因子：
6
作者：
Huang, Luping;Garcia, Gabriela;Sheikh-Hamad, David
通讯作者：
Sheikh-Hamad, David
DOI：
10.1073/pnas.1008117107
发表时间：
2010-08-03
影响因子：
11.1
作者：
Bartosh, Thomas J.;Ylostalo, Joni H.;Prockop, Darwin J.
通讯作者：
Prockop, Darwin J.
影响因子：
--
作者：
Mindrescu, C;Dias, AAM;Wisniewski, HG
通讯作者：
Wisniewski, HG