Differential expression and sequence analysis of the maize glyceraldehyde-3-phosphate dehydrogenase gene family.
Differential expression and sequence analysis of the maize glyceraldehyde-3-phosphate dehydrogenase gene family.
复制标题
DOI：
10.1105/tpc.1.8.793
复制
发表时间：
1989-08
期刊：
影响因子：
--
通讯作者：
D. A. Russell;M. Sachs
中科院分区：
文献类型：
--
作者：
D. A. Russell;M. Sachs
描述了玉米胞质甘油醛-3-磷酸脱氢酶的两个cDNA克隆。其中一个与先前发表的克隆在编码能力上约97%相似[Brinkmann et al.（1987）. J. Mol.评价26，320-328]，而另一个显示只有88%的相似性。有证据表明，这三种cDNA是三种基因的产物，称为Gpc 1、Gpc 2和Gpc 3。当使用对应于Gpc 3的最不相似的克隆来分析RNA凝胶印迹时，厌氧处理6小时诱导芽中的RNA积累15.6倍，而从28摄氏度到40摄氏度的1小时转变使积累增加5.1倍。根具有更高的基础表达水平，导致6.0倍的厌氧诱导和2.4倍的热应激诱导。使用对应于Gpc 2的克隆进行的RNA凝胶印迹分析显示，在厌氧6小时内RNA积累减少，而使用先前发表的对应于Gpc 1的克隆进行的分析显示，在24小时内RNA积累减少。Gpc 1和Gpc 2都没有表现出热胁迫诱导，而其他一些已知的厌氧基因则表现出热胁迫诱导。通过使用杂交选择、体外翻译和免疫沉淀，显示了三个基因的相对表达。所观察到的基因表达的变化的作用进行了讨论，有关应激生理。
Two cDNA clones for maize cytosolic glyceraldehyde-3-phosphate dehydrogenase are described. One is about 97% similar in coding capacity to a previously published clone [Brinkmann et al. (1987). J. Mol. Evol. 26, 320-328], while the other shows only 88% similarity. Evidence points toward the three cDNAs being the products of three genes, to be called Gpc1, Gpc2, and Gpc3. When the least similar clone, corresponding to Gpc3, was used to analyze RNA gel blots, anaerobic treatment for 6 hours induced RNA accumulation in the shoots 15.6-fold, while a 1-hour shift from 28 degrees C to 40 degrees C increased accumulation 5.1-fold. Roots had a higher basal level of expression, leading to a 6.0-fold anaerobic induction, and a 2.4-fold heat stress induction. RNA gel blot analysis using the clone corresponding to Gpc2 showed decreased RNA accumulation within 6 hours of anaerobiosis, while analysis with the previously published clone, corresponding to Gpc1, showed a decrease within 24 hours. Neither Gpc1 nor Gpc2 showed heat stress induction, while some other known anaerobic genes did. Through the use of hybrid selection, in vitro translation, and immune precipitation, the relative expression of the three genes is shown. The role of the observed changes in gene expression is discussed in relation to stress physiology.