Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage.
Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage.
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DOI：
10.1186/1472-6882-14-277
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发表时间：
2014-07-30
影响因子：
--
通讯作者：
Adewole SO
中科院分区：
文献类型：
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作者：
Adeyemi DO;Ukwenya VO;Obuotor EM;Adewole SO
研究了芙蓉花萼醇提物富黄酮类化合物水溶液组分对链脲佐菌素诱导的糖尿病大鼠的抗肝毒作用。用0.1 M柠檬酸缓冲液（pH 6.3）溶解链脲佐菌素80 mg/kg b.w.，单次腹腔注射诱导Wistar大鼠糖尿病。从组织病理学分析和血清和肝脏匀浆的生化指标评估中可以看出，提取物对stz -糖尿病诱导的肝损伤有明显的改善作用。木芙花处理后，糖尿病大鼠肝脏谷胱甘肽（GSH）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）水平（分别为3.76±0.38 μM、0.42±0.04 U/L、41.08±3.04 U/ml、0.82±0.04 U/L）降至接近正常水平（分别为6.87±0.51 μM、0.72±0.06 U/L、87.92±5.26 U/ml、1.37±0.06 U/L）。木槿治疗后，糖尿病大鼠血清中天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP）水平均恢复正常。糖尿病大鼠肝染色切片显示肝纤维化及糖原过多沉积。这些病理改变在提取物处理大鼠得到改善。木芙蓉提取物对STZ型糖尿病大鼠的抗肝毒作用可能与其抗氧化活性和黄酮类化合物的存在有关。
Flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats. Diabetes Mellitus was induced in Wistar rats by a single i.p injection of 80 mg/kg b.w. streptozotocin (STZ) dissolved in 0.1 M citrate buffer (pH 6.3). The ameliorative effects of the extract on STZ-diabetes induced liver damage was evident from the histopathological analysis and the biochemical parameters evaluated in the serum and liver homogenates. Reduced levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (3.76 ± 0.38 μM, 0.42 ± 0.04 U/L, 41.08 ± 3.04 U/ml, 0.82 ± 0.04 U/L respectively) in the liver of diabetic rats were restored to a near normal level in the Hibiscus sabdariffa -treated rats (6.87 ± 0.51 μM, 0.72 ± 0.06 U/L, 87.92 ± 5.26 U/ml, 1.37 ± 0.06 U/L respectively). Elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) in the serum of diabetic rats were also restored in Hibiscus sabdariffa -treated rats. Examination of stained liver sections revealed hepatic fibrosis and excessive glycogen deposition in the diabetic rats. These pathological changes were ameliorated in the extract-treated rats. The anti-hepatotoxic activity of Hibiscus sabdariffa extract in STZ diabetic rats could be partly related to its antioxidant activity and the presence of flavonnoids.