Natural cell‐mediated cytotoxicity in mice against non‐lymphoid tumor cells and some normal cells
Natural cell‐mediated cytotoxicity in mice against non‐lymphoid tumor cells and some normal cells
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小鼠体内针对非淋巴肿瘤细胞和一些正常细胞的天然细胞介导的细胞毒性
DOI：
10.1002/ijc.2910200309
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发表时间：
1977
影响因子：
6.4
通讯作者：
H. Holden
中科院分区：
文献类型：
--
作者：
M. Nunn;R. Herberman;H. Holden
在短期51 Cr释放试验中，发现正常小鼠的淋巴细胞对各种非淋巴肿瘤细胞以及淋巴瘤具有细胞介导的细胞毒性。一些非淋巴肿瘤与标准使用的淋巴系一样易受天然细胞毒性的影响。一些组织培养细胞系和体内传代肿瘤系易发生裂解，一些原发性病毒诱导的淋巴瘤也是如此。在裸鼠中产生的肿瘤具有高水平的天然细胞毒活性，对裂解都有抗性。除了转化细胞对天然细胞介导的细胞毒性的易感性外，一些未转化的培养细胞和来自正常组织的细胞也是该机制的靶细胞。在正常胸腺细胞、骨髓细胞和短期巨噬细胞培养物中观察到低水平的细胞毒性，而正常脾和淋巴结细胞对溶解完全耐受。这些结果表明，小鼠天然细胞介导的细胞毒性反应性比先前认识到的范围更广。
Lymphocytes from normal mice were found to have cell‐mediated cytotoxicity, in a short‐term 51Cr release assay, against a variety of non‐lymphoid tumor cells as well as against lymphomas. Some of the non‐lymphoid tumors were as susceptible to natural cytotoxicity as the standardly used lymphoid lines. Some tissue culture cell lines and in vivo passaged tumor lines were susceptible to lysis, as were some primary virus‐induced lymphomas. Tumors which arose in nude mice, which have high levels of natural cytotoxic activity, were all resistant to lysis. In addition to the susceptibility of transformed cells to natural cell‐mediated cytotoxicity, some untransformed cultured cells and cells from normal tissues were targets for this mechanism. Low levels of cytotoxicity were seen with normal thymus cells, bone‐marrow cells, and short term cultures of macrophages, whereas normal spleen and lymph‐node cells were completely resistant to lysis. These results indicate a broader spectrum for mouse natural cell‐mediated cytotoxicity reactivity than has been previously recognized.