The effect of glucose modification of hydroxyapatite nanoparticles on gene delivery
The effect of glucose modification of hydroxyapatite nanoparticles on gene delivery
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DOI：
10.1002/jbm.a.36523
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发表时间：
2019-01-01
影响因子：
4.9
通讯作者：
Nagai, Akiko
中科院分区：
文献类型：
--
作者：
Komuro, Hiroaki;Sasano, Tetsuo;Nagai, Akiko
表面修饰技术已被应用于生物相容性和生物可吸收的羟基磷灰石（HAp）纳米颗粒在细胞生物学和医学中用于传递生物活性分子。我们证明了葡萄糖修饰HAp （GlcHAp）对内皮细胞转染效率的影响。用微乳法制备均匀的HAp纳米颗粒后，将带或不带葡萄糖修饰的颗粒和质粒DNA （pDNA）复合物转染内皮细胞。GlcHAp/pDNA转染效率高于HAp/pDNA转染效率。为了阐明葡萄糖修饰后转染效率提高的机制，研究了进入细胞的摄取途径和对DNA降解的抑制作用。通过选择性抑制剂实验观察到，GlcHAp/pDNA在与葡萄糖转运蛋白1相互作用后提高了转染效率。此外，在DNA降解实验中，GlcHAp/pDNA比HAp/pDNA更稳定。结果表明，葡萄糖修饰可以促进细胞对HAp纳米颗粒的吸收，保护内化DNA；非病毒转染载体的基本特性。(c) 2018 Wiley期刊公司中国生物医学工程学报，2016,31(1):444 - 444。
Surface modification techniques have been employed for the use of biocompatible and bioresorbable hydroxyapatite (HAp) nanoparticles in cell biology and medicine for the delivery of bioactive molecules. We demonstrated the effects of glucose modification of HAp (GlcHAp) on the transfection efficiency in endothelial cells. After preparing homogeneous HAp nanoparticles with a microemulsion technique, the particles with or without glucose modification and plasmid DNA (pDNA) complexes were transfected into endothelial cells. The transfection efficiency of GlcHAp/pDNA was higher than that of HAp/pDNA. To elucidate the mechanism underlying the improvement in the transfection efficiency following glucose modification, the uptake route into the cells and the inhibition of DNA degradation were investigated. GlcHAp/pDNA enhanced the transfection efficiency after interacting with the glucose transporter 1, as observed by the selective inhibitor assay. In addition, GlcHAp/pDNA was more stable than HAp/pDNA in the DNA degradation assay. Our results suggest that the glucose modification could promote the uptake of HAp nanoparticles by cells and protect the internalized DNA; properties essential for non-viral transfection carriers. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 61-66, 2019.