Functional restoration of rabbit spinal cord using collagen‐filament scaffold
Functional restoration of rabbit spinal cord using collagen‐filament scaffold
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DOI：
10.1002/term.130
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发表时间：
2009-01
影响因子：
3.3
通讯作者：
S. Yoshii;S. Ito;M. Shima;A. Taniguchi;M. Akagi
中科院分区：
文献类型：
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作者：
S. Yoshii;S. Ito;M. Shima;A. Taniguchi;M. Akagi
我们报道了首次成功地用胶原纤维神经支架修复兔脊髓横断后的功能。我们将5 mm长的6000胶原纤维平行于脊髓轴线移植到21只成年兔脊髓3 mm的缺损处，另取18只兔作为对照。在39只兔子中，有22只活过了实验期。术后12周，6只兔中有4只再生轴突穿过近端脊髓-植入物界面。术后24周，6只兔中有4只再生轴突穿过近端和远端脊髓-植入物界面。术后24周，胶原纤维移植组BBB运动评定量表评分为4.7±2.3，对照组为2.8±0.5。移植组BBB量表评分明显优于对照组。结果提示，胶原-细丝神经支架与脊髓轴线平行移植，可促进脊髓轴突再生和功能恢复。功能恢复似乎是永久性的，这增加了在人类身上应用治疗的可能性。版权所有©2008 John Wiley&Sons，Ltd.
We report the first success of functional restoration of transected rabbit spinal cord using collagen‐filament nerve scaffold. We grafted 5 mm‐long 6000 collagen filaments parallel to the axis of the spinal cord to bridge 3 mm defects of 21 adult rabbit spinal cords; 18 rabbits were used as controls. Of the 39 rabbits, 22 survived the experimental period. At 12 weeks postoperatively, regenerated axons crossed the proximal spinal cord–implant interfaces in four out of six rabbits. At 24 weeks postoperatively, regenerated axons crossed the proximal and distal spinal cord–implant interfaces in four out of six rabbits. At 24 weeks postoperatively, the Basso–Beattie–Bresnahan (BBB) locomotor rating scale scores of the rabbits in the collagen‐filament grafted group were 4.7 ± 2.3, while the score in the control group was 2.8 ± 0.5. The BBB scale scores of the grafted group were significantly better than the control group. The results suggest that the collagen‐filament nerve scaffold supports the axonal regeneration of the transected spinal cord and the restoration of function when grafted parallel to the axis of the spinal cord. The functional restoration appeared to be permanent, raising the possibility of therapeutic application in humans. Copyright © 2008 John Wiley & Sons, Ltd.