Lipid bilayer disruption induced by amphiphilic Janus nanoparticles: the non-monotonic effect of charged lipids
Lipid bilayer disruption induced by amphiphilic Janus nanoparticles: the non-monotonic effect of charged lipids
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DOI：
10.1039/c8sm02525h
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发表时间：
2019-03-21
期刊：
影响因子：
3.4
通讯作者：
Yu, Yan
中科院分区：
文献类型：
--
作者：
Lee, Kwahun;Yu, Yan
在这项研究中，我们报告了复杂的影响，带电脂质的两亲性Janus纳米粒子和脂质双层之间的相互作用。Janus纳米颗粒在一个半球上是阳离子的，在另一个半球上是疏水的。我们发现，纳米粒子，超过阈值浓度，诱导空穴在阳离子和阴离子脂质双层主要由疏水相互作用驱动。然而，这些缺陷的形成是非单调依赖于离子脂质组合物。颗粒和阴离子脂质双层之间的静电吸引增强了颗粒吸附，降低了缺陷引发的颗粒浓度阈值，但导致更局部的膜破裂。静电排斥导致减少阳离子双层上的颗粒吸附和广泛的缺陷形成，峰值在中间含量的阳离子脂质。这项研究阐明了重要的作用，脂质组合物在影响如何两亲性Janus纳米粒子相互作用和干扰脂质膜。
In this study, we report the complex effects of charged lipids on the interaction between amphiphilic Janus nanoparticles and lipid bilayers. Janus nanoparticles are cationic on one hemisphere and hydrophobic on the other. We show that the nanoparticles, beyond threshold concentrations, induce holes in both cationic and anionic lipid bilayers mainly driven by hydrophobic interactions. However, the formation of these defects is non-monotonically dependent on ionic lipid composition. The electrostatic attraction between the particles and anionic lipid bilayers enhances particle adsorption and lowers the particle concentration threshold for defect initiation, but leads to more localized membrane disruption. Electrostatic repulsion leads to reduced particle adsorption on cationic bilayers and extensive defect formation that peaks at intermediate contents of cationic lipids. This study elucidates the significant role lipid composition plays in influencing how amphiphilic Janus nanoparticles interact with and perturb lipid membranes.