Microcomputed Tomographic and Histomorphometric Analyses of Novel Titanium Mesh Membranes for Guided Bone Regeneration: A Study in Rat Calvarial Defects
Microcomputed Tomographic and Histomorphometric Analyses of Novel Titanium Mesh Membranes for Guided Bone Regeneration: A Study in Rat Calvarial Defects
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DOI：
10.11607/jomi.3219
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发表时间：
2014-07-01
影响因子：
2
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Rakhmatia, Yunia Dwi;Ayukawa, Yasunori;Koyano, Kiyoshi
目的：本研究的目的是评估新型钛网膜的最佳厚度和孔隙率，以增强骨增量，防止软组织向内生长，并防止膜暴露。材料和方法：使用六种不同厚度和孔径的新型钛网以及三种市售膜来覆盖 6 周大 Sprague-Dawley 大鼠手术造成的颅骨缺损。 4或8周后将动物处死。进行显微计算机断层扫描分析以分析三维骨体积和骨矿物质密度。还通过组织学和组织形态计量学评估了软组织向内生长。结果：本研究中使用的新型钛膜在增强大鼠颅骨缺损模型中的骨量方面与市售膜一样有效。在具有较大孔的 100 微米厚的膜上观察到最大的骨体积，尽管这些膜促进了矿物质密度较低的骨的生长。当使用100μm膜时，软组织向内生长在4周时增加，但在8周时再次下降至与其他膜没有统计学显着差异的水平。结论：膜厚度影响新骨形成总量，膜孔隙率是引导骨再生的重要因素，尤其是在愈合初期，尽管最终获得的骨量基本相同。新开发的厚度为 100 μm、孔径较大的钛网膜似乎是引导骨再生的最佳选择。
Purpose: The objective of this study was to evaluate the optimal thickness and porosity of novel titanium mesh membranes to enhance bone augmentation, prevent soft tissue ingrowth, and prevent membrane exposure. Materials and Methods: Six types of novel titanium meshes with different thicknesses and pore sizes, along with three commercially available membranes, were used to cover surgically created calvarial defects in 6-week-old Sprague-Dawley rats. The animals were killed after 4 or 8 weeks. Microcomputed tomographic analyses were performed to analyze the three-dimensional bone volume and bone mineral density. Soft tissue ingrowth was also evaluated histologically and histomorphometrically. Results: The novel titanium membranes used in this study were as effective at augmenting bone in the rat calvarial defect model as the commercially available membranes. The greatest bone volume was observed on 100-mu m-thick membranes with larger pores, although these membranes promoted growth of bone with lower mineral density. Soft tissue ingrowth when 100-mu m membranes were used was increased at 4 weeks but decreased again by 8 weeks to a level not statistically significantly different from other membranes. Conclusion: Membrane thickness affects the total amount of new bone formation, and membrane porosity is an essential factor for guided bone regeneration, especially during the initial healing period, although the final bone volume obtained is essentially the same. Newly developed titanium mesh membranes of 100 mu m in thickness and with large pores appear to be optimal for guided bone regeneration.