Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to Elimination of Germline-Specific DNA.
Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to Elimination of Germline-Specific DNA.
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DOI：
10.1016/j.celrep.2017.06.050
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发表时间：
2017-07-11
期刊：
影响因子：
8.8
通讯作者：
Nowacki M
中科院分区：
文献类型：
--
作者：
Furrer DI;Swart EC;Kraft MF;Sandoval PY;Nowacki M
Piwi蛋白和piRNAs保护真核生殖系免受转座子的传播。在纤毛虫草履虫的发育过程中，两个依赖Piwi的sRNA类参与了转座子和转座子衍生DNA的消除：与Ptiwi01和Ptiwi09相关的扫描RNAs(ScnRNAs)和iesRNAs，我们现在确定其结合伙伴为Ptiwi10和Ptiwi11。ScnRNAs来自母体基因组，在发育过程中启动DNA消除，而iesRNAs继续DNA靶向，直到去除过程完成。在这里，我们证明了scnRNAs和iesRNAs是由不同的Diller样蛋白处理的，并以一种相互排斥的方式与Piwi蛋白结合，这表明了不同的生物发生途径。我们还证明了PTIWI10基因是从发育中的细胞核转录而来的，它的转录依赖于先前的DNA切除，这表明了一种通过移除干扰基因的短DNA片段来触发基因表达调控的机制。鉴定了两个与iesRNAs相关的Piwi蛋白(Ptiwi10/11)。Piwi蛋白结合DICER产生的sRNA并去除客体链Ptiwi10是从新的体细胞大核中表达的DNA消除激活了基因转录因子等。结果表明，在草履虫中，两个小的RNA类别被不同的Piwi蛋白结合，以介导两个功能上分离的DNA消除途径。
Piwi proteins and piRNAs protect eukaryotic germlines against the spread of transposons. During development in the ciliate Paramecium, two Piwi-dependent sRNA classes are involved in the elimination of transposons and transposon-derived DNA: scan RNAs (scnRNAs), associated with Ptiwi01 and Ptiwi09, and iesRNAs, whose binding partners we now identify as Ptiwi10 and Ptiwi11. scnRNAs derive from the maternal genome and initiate DNA elimination during development, whereas iesRNAs continue DNA targeting until the removal process is complete. Here, we show that scnRNAs and iesRNAs are processed by distinct Dicer-like proteins and bind Piwi proteins in a mutually exclusive manner, suggesting separate biogenesis pathways. We also demonstrate that the PTIWI10 gene is transcribed from the developing nucleus and that its transcription depends on prior DNA excision, suggesting a mechanism of gene expression control triggered by the removal of short DNA segments interrupting the gene. Identification of two Piwi proteins (Ptiwi10/11) associated with iesRNAs Piwi proteins bind Dicer-produced sRNAs and remove passenger strands Ptiwi10 is expressed from the new somatic macronucleus DNA elimination activates gene transcription Furrer et al. show that two small RNA classes are bound by different Piwi proteins to mediate two functionally separate DNA elimination pathways in Paramecium.
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