Perturbation of anion balance during inhibition of growth of Escherichia coli by weak acids
Perturbation of anion balance during inhibition of growth of Escherichia coli by weak acids
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DOI：
10.1128/jb.180.4.767-772.1998
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发表时间：
1998-02-01
影响因子：
3.2
通讯作者：
Booth, IR
中科院分区：
文献类型：
--
作者：
Roe, AJ;McLaggan, D;Booth, IR
在弱酸抑制细胞生长的过程中，细胞质中有大量的弱酸阴离子积累。本研究旨在确定阴离子积累对细胞池的影响。在pH为6时，8 mM醋酸盐的存在导致细胞内形成了一个大于240 mM的醋酸阴离子池，导致细胞内谷氨酸水平降低，但K+和Na+水平没有显著变化。在低渗透压下，谷氨酸池的变化只补偿了积累的乙酸阴离子的一小部分。然而，在高渗透压下，谷氨酸补偿了积累的醋酸盐的一半以上。去除乙酸后恢复正常的细胞质pH依赖于谷氨酸的合成。
During inhibition of cell growth by weak acids, there is substantial accumulation of the weak acid anions in the cytoplasm. This study was undertaken to determine the impact of anion accumulation on cellular pools. At pH 6, growth in the presence of 8 mM acetate led to an internal pool of greater than 240 mM acetate anion and resulted in reduced levels of glutamate in the cell, but there were no significant changes in K+ and Na+ levels. At low osmolarity, the change in the glutamate pool compensated for only a small fraction of the accumulated acetate anion. However, at high osmolarity, glutamate compensated for over half of the accumulated acetate. Recovery of the normal cytoplasmic pH after the removal of acetate was dependent on the synthesis of glutamate.