6PPD-Quinone: Revised Toxicity Assessment and Quantification with a Commercial Standard
6PPD-Quinone: Revised Toxicity Assessment and Quantification with a Commercial Standard
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DOI：
10.1021/acs.estlett.1c00910
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发表时间：
2022-01-11
影响因子：
10.9
通讯作者：
Kolodziej, Edward P.
中科院分区：
文献类型：
--
作者：
Tian, Zhenyu;Gonzalez, Melissa;Kolodziej, Edward P.
雨水暴露可导致银鲑急性死亡，而6PPD-醌（6PPD-Q）被确定为主要的致病毒物。6PPDQ的商业标准最近已经可用;他们的分析强调了先前关于6PPD-Q的报告中的系统性高偏倚。使用6PPD-Q商业标准品重新确认幼银鲑中的毒性估计值，并开发用于定量的液相色谱-串联质谱分析方法。商业标准品的峰面积响应值比内部标准品高15倍，更新的LC 50值（95 ng/L）比先前报告的值低8.3倍。这些数据支持先前对发生率和毒性的相对比较，同时证实了6PPD-Q的实质性致死性。虽然环境浓度预计会较低，但6 PPD-Q的毒性也比以前计算的更大，应被归类为对水生生物“毒性极高”的污染物。同位素稀释-串联质谱法使得能够进行准确的定量（同位素稀释-串联质谱法的定量限）。
Stormwater exposure can cause acute mortality of coho salmon (Oncorhynchus kisutch), and 6PPD-quinone (6PPD-Q) was identified as the primary causal toxicant. Commercial standards of 6PPDQ recently became available; their analysis highlighted a systematic high bias in prior reporting concerning 6PPD-Q. A 6PPD-Q commercial standard was used to re-confirm toxicity estimates in juvenile coho salmon and develop a liquid chromatography-tandem mass spectrometry analytical method for quantification. Peak area responses of the commercial standard were similar to 15 times higher than those of in-house standards, and the updated LC50 value (95 ng/L) was similar to 8.3-fold lower than that previously reported. These data support prior relative comparisons of the occurrence and toxicity while confirming the substantial lethality of 6PPD-Q. While environmental concentrations are expected to be lower, 6PPD-Q also was more toxic than previously calculated and should be categorized as a "very highly toxic" pollutant for aquatic organisms. Isotope dilution-tandem mass spectrometry methods enabled accurate quantification (limits of quantification of