Cytotoxic evaluation of arsenic compounds in alveolar macrophages in hamsters
Cytotoxic evaluation of arsenic compounds in alveolar macrophages in hamsters
复制标题
仓鼠肺泡巨噬细胞中砷化合物的细胞毒性评价
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Kotani
中科院分区：
文献类型：
--
作者：
M. Okada;Y. Inoue;H. Karube;M. Niitsuya;Hideki Tohnori;Y. Aizawa;I. Okayasu;M. Kotani
砷化镓（GaAs）在动物中引起各种毒性作用，包括肺部疾病。虽然它们的毒性尚未完全调查，但GaAs被用于各种半导体产品的工作场所。采用磁法、酶释放试验和形态学观察等方法，研究了GaAs混悬液和AsCl3溶液对仓鼠肺泡巨噬细胞的毒性作用。将通过气管支气管灌洗从仓鼠中获得并粘附在威尔斯底部圆盘上的肺泡巨噬细胞暴露于四氧化三铁和添加剂。对四氧化三铁颗粒进行了磁化处理，并测量了磁化磁场。由于暴露于GaAs悬浮液，从肺泡巨噬细胞辐射的弛豫，快速下降的reflux磁场，延迟和衰减常数呈剂量依赖性下降。由于暴露在AsCl3溶液中，弛豫被大大延迟，衰减常数大大降低。由于这种松弛被认为与细胞骨架有关，因此暴露于GaAs会损害它们的运动功能。酶释放试验和形态学结果表明巨噬细胞损伤。因此，细胞毒性引起细胞结构变化和细胞死亡。根据DNA电泳和TUNEL方法，坏死变化比凋亡变化更频繁地发生。可见，GaAs悬液和AsCl3溶液对肺泡巨噬细胞有明显的细胞毒性作用.
Gallium arsenide (GaAs) causes various toxic effects in animals, including pulmonary diseases. Although their toxicity is not completely investigated, GaAs is used in workplaces in various semiconductor products. The present report was designed to clarify the toxicity of GaAs suspension and arsenic chloride (AsCl 3 ) solution as additives to the alveolar macrophages in hamsters using magnetometry, enzyme release assays and morphological examinations. Alveolar macrophages obtained from hamsters by tracheobronchial lavage and adhered to disks in the bottom of wells were exposed to ferrosoferric oxide and additives. Ferrosoferric oxide particles were magnetized externally and the remanent magnetic field was measured. Relaxation, a fast decline of remanent magnetic fields radiated from alveolar macrophages, was delayed and decay constants decreased dose-dependently due to exposure to GaAs suspensions. Relaxation was much delayed and decay constants decreased considerably due to exposure to AsCl 3 solutions. Because the relaxation is thought to be associated with the cytoskeleton, exposure to GaAs impairs their motor function. Enzyme release assay and morphological findings indicated damage to macrophages. Thus the cytotoxicity caused cytostructural changes and cell death. According to DNA electrophoresis and TUNEL methods, necrotic changes occur more frequently than apoptotic changes. In conclusion, exposure to GaAs suspension and AsCl 3 solution induced cytotoxicity of alveolar macrophages.
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影响因子：
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作者：
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