tRNA cleavage is a conserved response to oxidative stress in eukaryotes
tRNA cleavage is a conserved response to oxidative stress in eukaryotes
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DOI：
10.1261/rna.1232808
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发表时间：
2008-10-01
期刊：
影响因子：
4.5
通讯作者：
Parker, Roy
中科院分区：
文献类型：
--
作者：
Thompson, Debrah M.;Lu, Cheng;Parker, Roy
最近的结果已经在广泛的生物体中鉴定出了小RNA的多样性。在这项工作中，我们证明，酿酒酵母含有一个小的RNA人口主要由tRNA的一半和rRNA片段。通过北方印迹分析可检测到tRNA的5'和3'片段，表明存在核酸内切裂解过程。酵母中tRNA和rRNA片段的产生在氧化应激条件下最明显，特别是在进入稳定期期间。类似的tRNA片段也观察到在人类细胞系和植物在氧化应激。这些结果表明，tRNA切割是氧化应激反应的保守方面。
Recent results have identified a diversity of small RNAs in a wide range of organisms. In this work, we demonstrate that Saccharomyces cerevisiae contains a small RNA population consisting primarily of tRNA halves and rRNA fragments. Both 5' and 3' fragments of tRNAs are detectable by Northern blot analysis, suggesting a process of endonucleolytic cleavage. tRNA and rRNA fragment production in yeast is most pronounced during oxidative stress conditions, especially during entry into stationary phase. Similar tRNA fragments are also observed in human cell lines and in plants during oxidative stress. These results demonstrate that tRNA cleavage is a conserved aspect of the response to oxidative stress.