A targeted metabolomics approach to understand differences in flavonoid biosynthesis in red and yellow raspberries
A targeted metabolomics approach to understand differences in flavonoid biosynthesis in red and yellow raspberries
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DOI：
10.1016/j.plaphy.2013.04.001
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发表时间：
2013-11-01
影响因子：
6.5
通讯作者：
Martens, Stefan
中科院分区：
文献类型：
--
作者：
Carvalho, Elisabete;Franceschi, Pietro;Martens, Stefan
酚类化合物是树莓中最重要的一类次生代谢产物，在植物中具有广泛的生物学功能。由于它们存在于水果中，它们也被认为是人类营养中重要的生物活性化合物，与水果品质密切相关。本研究采用UPLC-MS/MS方法对红树莓和黄树莓果实中的酚类化合物进行了筛选。总共检测到50种酚类化合物高于定量限。除了明显缺乏花青素，这里分析的所有黄色水果都缺乏原花青素B1。这种二聚体的存在下，沿着与B3二聚体描述为第一次在树莓果实。也是第一次，二氢查耳酮和芪衍生物和槲皮素代谢产物，异鼠李素及其糖苷，被确定在相当大的浓度在树莓。基于PCA图，红色品种“Heritage”和黄色“Alpen Gold”由于其不同的代谢物谱/浓度而可以与其他测试的品种清楚地分开。这项研究允许获得一个全面的配置文件的不同树莓品种的酚类成分。所获得的数据将导致更好地了解乌藨子中多酚的整体生物合成网络，并将有助于解释正在进行的研究中不同代谢产物谱的责任因素。(C)2013年Elsevier Masson SAS。All rights reserved.
Phenolic compounds account for the most important class of secondary metabolites in raspberries and fulfill a broad range of biological functions in plants. Due to their presence in fruits they are also considered as important bioactive compounds in human nutrition and are closely related to fruit quality. In the present study a targeted UPLC-MS/MS method was used to screen various phenolic compounds in fruits of red and yellow raspberry cultivars. In total 50 phenolic compounds were detected above the quantification limit. Beside the obvious lack of anthocyanins, all yellow fruits analysed here lack procyanidin B1. The presence of this dimer, along with B3 dimers is described for the first time in raspberry fruits. Also for the first time, dihydrochalcone and stilbene derivatives and the quercetin metabolite, isorhamnetin with its glycosides, were identified in considerable concentrations in raspberries. Based on a PCA plot the red cultivar "Heritage" and the yellow "Alpen Gold" could clearly be separated from the other tested cultivars due to their distinct metabolite profiles/concentrations. This study allowed to obtain a comprehensive profile of the phenolic composition of the different raspberry varieties. The obtained data will lead to a better understanding of the overall biosynthetic network of polyphenols in raspberry and will help to explain responsible factors for the different metabolite profiles in ongoing studies. (C) 2013 Elsevier Masson SAS. All rights reserved.