Near-infrared (NIR) controlled reversible cell adhesion on a responsive nano-biointerface
Near-infrared (NIR) controlled reversible cell adhesion on a responsive nano-biointerface
复制标题
近红外 (NIR) 控制的响应性纳米生物界面上的可逆细胞粘附
DOI：
10.1007/s12274-017-1446-1
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发表时间：
2017-04-01
期刊：
影响因子：
9.9
通讯作者：
Wang, Shutao
中科院分区：
文献类型：
--
作者：
Cui, Haijun;Zhang, Pengchao;Wang, Shutao
光激活的动态变化促进了智能接口，特别是纳米生物接口的发展。在本文中，通过将热响应聚合物（聚（N-异丙基丙烯酰胺），PNIPAM）接枝到硅纳米线（SiNW）而不是传统的光敏部分上，设计了用于控制细胞粘附的近红外（NIR）响应表面。近红外诱发 SiNW 的光热效应，以及局部热诱发 PNIPAM 的热力学相变。随着近红外辐射的应用，表面转变为疏水状态，并在关闭近红外辐射时恢复到亲水状态，从而导致可逆的细胞粘附和释放。即使在 20 个循环后，表面的可切换润湿性和细胞粘附/释放也有效发生。使用近红外光谱通过疏水相互作用将蛋白质锚定在表面；细胞捕获剂的进一步连接有助于实现特定的细胞捕获。这种通过近红外对细胞粘附的动态控制可能为设计功能性纳米生物界面提供新的线索。
Light-activated dynamic variations have promoted the development of smart interfaces, especially nano-biointerfaces. In this article, the near-infrared (NIR)-responsive surface for controlling cell adhesion was designed by grafting a thermal responsive polymer (poly(N-isopropylacrylamide), PNIPAM) onto silicon nanowires (SiNWs) instead of the traditional photosensitive moieties. NIR induced the photothermal effect of the SiNWs, and the local heat induced thermodynamic phase transformation of PNIPAM. With the application of NIR radiation, the surface turned to a hydrophobic state, and restored to the hydrophilic state when NIR was switched off, leading to reversible cell adhesion and release. The switchable wettability of the surface and cell adhesion/release occurred efficiently even after 20 cycles. Proteins were anchored on the surface via hydrophobic interactions using NIR; further connection of a cell-capture agent helped in achieving specific cell capture. This dynamic control of cell adhesion via NIR may provide new clues for designing functional nano-biointerfaces.