Genetic architecture of glucosinolate variation in Brassica napus
Genetic architecture of glucosinolate variation in Brassica napus
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DOI：
10.1016/j.jplph.2019.06.001
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发表时间：
2019-09-01
影响因子：
4.3
通讯作者：
Bancroft, Ian
中科院分区：
文献类型：
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作者：
Kittipol, Varanya;He, Zhesi;Bancroft, Ian
硫代葡萄糖苷（GSL）水解产物具有多种生物活性，对油菜的防御系统和营养品质具有重要的生物学和经济学意义。然而，基于基因组的B.由于多倍体物种的复杂性，油菜GSL调节机制是稀缺的。为了解决这些挑战，我们使用了基于转录组的GWAS方法，关联转录组学（AT），跨越288个B的多样性小组。揭示控制B群体GSL数量变异的潜在遗传基础。油菜营养组织单核苷酸多态性（SNP）标记和基因表达标记（GEM）关联鉴定MYB 28/HAG 1（AT 5G 61420）的直向同源物，特别是染色体A9和C2上的拷贝，是叶片中脂肪族GSL变异的关键调节因子。我们发现，脂肪族GSL在种子和叶之间的正相关性是由于合成的量，控制Bna.HAG1.A9和Bna.HAG1.C2，而不是通过运输过程中的变化。此外，根组织中的AT和差异表达分析暗示MYB 29/HAG 3（AT 5G 07690）的直向同源物Bna. HAG 3. A3控制根芳香GSL变异。基于根表达数据，我们还提出Bna. MAM3.A3在控制苯丙氨酸链延长以用于芳香族GSL生物合成中具有作用。这项工作揭示了高苯丙氨酸衍生的芳香族GSL的调节剂，并暗示脂肪族和芳香族GSL之间共享的生物合成途径。
The diverse biological activities of glucosinolate (GSL) hydrolysis products play significant biological and economical roles in the defense system and nutritional qualifies of Brassica napus (oilseed rape). Yet, genomic-based study of the B. napus GSL regulatory mechanisms are scarce due to the complexity of working with polyploid species. To address these challenges, we used transcriptome-based GWAS approach, Associative Transcriptomics (AT), across a diversity panel of 288 B. napus genotypes to uncover the underlying genetic basis controlling quantitative variation of GSLs in B. napus vegetative tissues. Single nucleotide polymorphism (SNP) markers and gene expression markers (GEMs) associations identify orthologues of MYB28/HAG1 (AT5G61420), specifically the copies on chromosome A9 and C2, to be the key regulators of aliphatic GSL variation in leaves. We show that the positive correlation observed between aliphatic GSLs in seed and leaf is due to the amount synthesized, as controlled by Bna.HAG1.A9 and Bna.HAG1.C2, rather than by variation in the transport processes. In addition, AT and differential expression analysis in root tissues implicate an orthologue of MYB29/HAG3 (AT5G07690), Bna.HAG3.A3, as controlling root aromatic GSL variation. Based on the root expression data we also propose Bna.MAM3.A3 to have a role in controlling phenylalanine chain elongation for aromatic GSL biosynthesis. This work uncovers a regulator of homophenylalanine-derived aromatic GSLs and implicates the shared biosynthetic pathways between aliphatic and aromatic GSLs.