Mutations affecting transcription pausing in the Bacillus subtilis pyr operon.
Mutations affecting transcription pausing in the Bacillus subtilis pyr operon.
复制标题
影响枯草芽孢杆菌pyr操纵子转录暂停的突变。
DOI：
10.1007/s00203-005-0020-5
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发表时间：
2005
影响因子：
2.8
通讯作者：
Switzer,RobertL
中科院分区：
文献类型：
--
作者：
Zhang,Hesheng;Jorgensen,CasperMoller;Switzer,RobertL
Pausing during transcription of theBacillus subtilis pyroperon was proposed to play a role in its regulation by attenuation. Substitution mutations in theB. subtilis pyrDNA specifying the 3′-terminal nucleotides of the previously identified transcription pause sites substantially reduced pausing at these sites in vitro. This result confirms the general utility of this mutagenic strategy for studying transcriptional pausing. Pyrimidine-mediated repression in vivo ofpyr-lacZfusions containing some of these substitution mutations was substantially lower than those observed with the wild-typepyr-lacZfusions. However, these defects in regulation were correlated with alterations in the stability of the terminator stem-loop specified by the attenuator, rather than with their effects on transcriptional pausing in vitro.
