Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant.
Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant.
复制标题
CHO Cdr 变体中细胞内金属硫蛋白浓度、锌或镉流入速率以及 MT mRNA 积累。
DOI：
10.1016/0014-4827(89)90046-3
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发表时间：
1989
影响因子：
3.7
通讯作者：
Huang,PC
中科院分区：
文献类型：
--
作者：
Morris,S;Huang,PC
研究了高表达金属硫蛋白（MT）的抗镉CHO细胞系中MT基因表达的调控。我们的研究结果表明，MT mRNA水平达到最高24-30小时后，初级锌暴露，随后，MT mRNA浓度下降。MT mRNA水平的降低可能与金属硫蛋白的积累和锌、镉吸收率的降低有关。
The regulation of metallothionein (MT) gene expression in a cadmium resistant CHO cell line which overproduces MT was examined in this study. Our results show that MT mRNA levels reach a maximum 24–30 h after a primary zinc exposure and, subsequently, MT mRNA concentrations decline. This decrease in MT mRNA levels can be correlated with the accumulation of metallothionein and decreased rates of zinc and cadmium uptake.