The role of the TH1 and TH2 immune responses in loosening and osteolysis of cemented total hip replacements
The role of the TH1 and TH2 immune responses in loosening and osteolysis of cemented total hip replacements
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DOI：
10.1002/jbm.a.10200
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发表时间：
2003-03-15
影响因子：
4.9
通讯作者：
Goodman, S
中科院分区：
文献类型：
--
作者：
Arora, A;Song, Y;Goodman, S
骨溶解和无菌性松动的发生机制影响全髋关节置换术(THR)的寿命。本研究检测了TH1和TH2亚群中的淋巴细胞在THR的骨溶解和无菌性松动中的特殊作用。来自松动、骨水泥髋臼假体患者假体周围区域的组织被用来测定TH1和TH2细胞因子谱。在翻修手术中采集了12例有骨溶解征象的患者的组织标本，以及9例无骨溶解迹象的患者的组织标本。冰冻切片用抗CD3、干扰素-γ、白介素2、白介素4和白介素10的一抗进行免疫组织化学染色，以确定每个切片的阳性细胞百分比。溶骨性组织与非溶骨性组织相比，TH1途径(干扰素-γ、IL-2)或TH2途径(IL-4、IL-10)阳性细胞百分率差异无统计学意义。然而，大量的T细胞(平均约占总细胞的10%)以及TH1和TH2免疫细胞因子(平均占细胞总数的3%-5%)可能在假体界面的免疫过程中发挥作用。(C)2003年威利期刊公司。
The mechanisms underlying the development of osteolysis and aseptic loosening have an impact on the longevity of total hip replacements (THRs). This study examines the specific roles of lymphocytes in the TH1 and TH2 subsets in osteolysis and aseptic loosening of THR. Tissue from periprosthetic regions from patients with loose, cemented acetabular components were used to determine the TH1 and TH2 cytokine profile. Twelve tissue specimens from patients with radiographic signs of osteolysis, and nine tissue specimens from patients with no signs of osteolysis were harvested during revision surgery. Immunohistochemistry using primary antibodies against CD3, interferon (IFN)-gamma, interleukin (IL)-2, IL-4, and IL-10 was performed on frozen sections to determine the percentage of positive cells for each of the sections. No statistically significant differences in the percentage of positive cells expressing cytokines characteristic of the TH1 pathway (IFN-gamma, IL-2) or TH2 pathway (IL-4, IL-10) were found when comparing osteolytic and non-osteolytic tissues. However, significant numbers of T cells (averaging about 10% of the total cells) and TH1 and TH2 immune cytokines (averaging 3-5% of cells) implicate a possible role for immune processes at the prosthetic interface. (C) 2003 Wiley Periodicals, Inc.